Department of Burns, Heilongjiang Provincial Hospital, Zhongshan Road, Harbin, Heilongjiang 150036, China.
Science and Education Department, Research Center for Family Planning of Guangxi Zhuang Autonomous Region, China.
J Photochem Photobiol B. 2018 Mar;180:253-258. doi: 10.1016/j.jphotobiol.2018.02.018. Epub 2018 Feb 16.
In the Present-day medicinally applied wound bandages have many drawbacks for, instance, rigidity, non-porosity, low mechanical potency, also an affinity for bandages to stick onto the injury exterior; additionally, a greater part of the bandages did not secure bactericidal activity. Hydrogel derived injury bandages would be supportive to afford a chill feeling with a humidity atmosphere, in addition, to performing as an obstruction to bacteria. To overcome these drawbacks, we have fabricated porous keratin-chitosan/n-ZnO nanocomposite (KCBZNs) bandages via the inclusion of nano-ZnO into the keratin-chitosan hydrogel. The functional group and surface of as-fabricated bandages were characterized using Fourier transform infrared spectroscopy (FT-IR), X-ray diffractometry (XRD), and scanning electron microscopy with energy-dispersive X-ray spectroscopy (SEM-EDX) and transmission electron microscopy with selected area diffraction (TEM-SAD). Moreover, mechanical, swelling, bactericidal, bio-compatibility of nanocomposite was assessed to exhibit its efficacy for biological applications. The nanocomposite illustrated increased swelling, and bactericidal activity. Bio-compatibility of the nanocomposite has been investigated in normal human fibroblast cells. Also, the in vivo assessments in SD rats exposed that as-fabricated nanocomposite bandages increased the wound curing with assisted for quicker skin cell construction along with collagen development. Hence, the acquired information strongly supports to utilize of this nanocomposite hydrogels for burn wounds.
目前，医用创可贴存在许多缺点，例如刚性、非透气性、机械强度低，以及创可贴容易粘在伤口外部；此外，大部分创可贴都没有杀菌活性。水凝胶制成的创可贴可以提供凉爽的感觉和潮湿的环境，同时还可以阻止细菌。为了克服这些缺点，我们通过将纳米氧化锌掺入角蛋白-壳聚糖水凝胶中，制备了多孔角蛋白-壳聚糖/纳米氧化锌（KCBZNs）复合创可贴。使用傅里叶变换红外光谱（FT-IR）、X 射线衍射（XRD）、带有能量色散 X 射线光谱（SEM-EDX）的扫描电子显微镜和带有选区衍射（TEM-SAD）的透射电子显微镜对制备的创可贴的官能团和表面进行了表征。此外，还评估了纳米复合材料的机械性能、溶胀性能、杀菌性能和生物相容性，以展示其在生物应用中的功效。纳米复合材料表现出了增加的溶胀性和杀菌活性。对纳米复合材料的生物相容性已在正常人类成纤维细胞中进行了研究。此外，在 SD 大鼠中的体内评估表明，所制备的纳米复合创可贴绷带可促进伤口愈合，有助于更快地构建皮肤细胞和胶原蛋白的生成。因此，这些研究结果有力地支持了将这种纳米复合水凝胶用于烧伤伤口。
