Cavazza Christine, Collin-Faure Véronique, Pérard Julien, Diemer Hélène, Cianférani Sarah, Rabilloud Thierry, Darrouzet Elisabeth
Univ. Grenoble Alpes, CEA, CNRS, IRIG, CBM, F-38000 Grenoble, France.
Laboratoire de Spectrométrie de Masse BioOrganique (LSMBO), Université de Strasbourg, CNRS, IPHC UMR 7178, 67000 Strasbourg, France; Infrastructure Nationale de Protéomique ProFI - FR2048 (CNRS-CEA), 67087 Strasbourg, France.
J Proteomics. 2022 Jan 6;250:104389. doi: 10.1016/j.jprot.2021.104389. Epub 2021 Sep 30.
Some carboxydotrophs like Rhodospirillum rubrum are able to grow with CO as their sole source of energy using a Carbone monoxide dehydrogenase (CODH) and an Energy conserving hydrogenase (ECH) to perform anaerobically the so called water-gas shift reaction (WGSR) (CO + HO → CO + H). Several studies have focused at the biochemical and biophysical level on this enzymatic system and a few OMICS studies on CO metabolism. Knowing that CO is toxic in particular due to its binding to heme iron atoms, and is even considered as a potential antibacterial agent, we decided to use a proteomic approach in order to analyze R. rubrum adaptation in term of metabolism and management of the toxic effect. In particular, this study allowed highlighting a set of proteins likely implicated in ECH maturation, and important perturbations in term of cofactor biosynthesis, especially metallic cofactors. This shows that even this CO tolerant microorganism cannot avoid completely CO toxic effects associated with its interaction with metallic ions. SIGNIFICANCE: This proteomic study highlights the fact that even in a microorganism able to handle carbon monoxide and in some way detoxifying it via the intrinsic action of the carbon monoxide dehydrogenase (CODH), CO has important effects on metal homeostasis, metal cofactors and metalloproteins. These effects are direct or indirect via transcription regulation, and amplified by the high interdependency of cofactors biosynthesis.
一些羧基营养菌，如深红红螺菌，能够以一氧化碳作为唯一能量来源生长，利用一氧化碳脱氢酶（CODH）和能量保守氢化酶（ECH）在厌氧条件下进行所谓的水煤气变换反应（WGSR）（CO + H₂O → CO₂ + H₂）。几项研究聚焦于该酶系统的生化和生物物理层面，以及一些关于CO代谢的组学研究。鉴于CO具有毒性，特别是由于其与血红素铁原子结合，甚至被视为一种潜在的抗菌剂，我们决定采用蛋白质组学方法来分析深红红螺菌在代谢和毒性效应管理方面的适应性。具体而言，这项研究揭示了一组可能与ECH成熟有关的蛋白质，以及辅因子生物合成方面的重要扰动，尤其是金属辅因子。这表明，即使是这种耐CO的微生物也无法完全避免与金属离子相互作用所带来的CO毒性效应。意义：这项蛋白质组学研究凸显了这样一个事实，即即使在一种能够处理一氧化碳并通过一氧化碳脱氢酶（CODH）的内在作用以某种方式对其进行解毒的微生物中，CO对金属稳态、金属辅因子和金属蛋白仍有重要影响。这些影响通过转录调控直接或间接产生，并因辅因子生物合成的高度相互依赖性而被放大。
