Bonam D, Lehman L, Roberts G P, Ludden P W
Department of Biochemistry, University of Wisconsin, Madison 53706-1569.
J Bacteriol. 1989 Jun;171(6):3102-7. doi: 10.1128/jb.171.6.3102-3107.1989.
Exposure of the photosynthetic bacterium Rhodospirillum rubrum to carbon monoxide led to increased carbon monoxide dehydrogenase and hydrogenase activities due to de novo protein synthesis of both enzymes. Two-dimensional gels of [35S]methionine-pulse-labeled cells showed that induction of CO dehydrogenase synthesis was rapidly initiated (less than 5 min upon exposure to CO) and was inhibited by oxygen. Both CO dehydrogenase and the CO-induced hydrogenase were inactivated by oxygen in vivo and in vitro. In contrast to CO dehydrogenase, the CO-induced hydrogenase was 95% inactivated by heating at 70 degrees C for 5 min. Unlike other hydrogenases, this CO-induced hydrogenase was inhibited only 60% by a 100% CO gas phase.
光合细菌红螺菌暴露于一氧化碳中会导致一氧化碳脱氢酶和氢化酶活性增加，这是由于这两种酶的从头蛋白质合成。用[35S]甲硫氨酸脉冲标记细胞的二维凝胶显示，一氧化碳脱氢酶合成的诱导迅速启动（暴露于一氧化碳后不到5分钟），并受到氧气的抑制。一氧化碳脱氢酶和一氧化碳诱导的氢化酶在体内和体外均被氧气灭活。与一氧化碳脱氢酶不同，一氧化碳诱导的氢化酶在70℃加热5分钟后95%被灭活。与其他氢化酶不同，这种一氧化碳诱导的氢化酶仅被100%的一氧化碳气相抑制60%。
