Soper B D, Tepperman B L
Prostaglandins. 1986 May;31(5):887-98. doi: 10.1016/0090-6980(86)90021-3.
In the present study we have investigated the macromolecular nature of porcine oxyntic mucosal PGE2 binding sites and the involvement of specific functional groups in the binding interaction. Incubation of oxyntic mucosal membranes with DNAse or RNAse did not influence binding. Phospholipase A2 was strongly inhibitory while phospholipases C and D exerted variable effects. Trypsinization of the membranes also reduced binding and this reduction was prevented by addition of soybean trypsin inhibitor. Neuraminidase and beta-galactosidase treatments resulted in variable increases in binding activity. The increase in binding was due to an increase in binding affinity and/or binding site concentration. Protein modifying reagents acetic anhydride, N-ethylmaleimide and mercaptoethanol all reduced binding. These results suggest the importance of protein, lipid and carbohydrate components of the membrane in the binding interaction between PGE2 and its binding site. The ability of mercaptoethanol and N-ethylmaleimide to reduce binding suggest the involvement of both sulphydryl and disulphide groups in the PGE2 binding reaction.
在本研究中，我们研究了猪胃黏膜中前列腺素E2（PGE2）结合位点的大分子性质以及特定官能团在结合相互作用中的参与情况。用脱氧核糖核酸酶（DNAse）或核糖核酸酶（RNAse）孵育胃黏膜膜不会影响结合。磷脂酶A2具有强烈的抑制作用，而磷脂酶C和D则产生不同的影响。对膜进行胰蛋白酶处理也会降低结合，而添加大豆胰蛋白酶抑制剂可防止这种降低。神经氨酸酶和β-半乳糖苷酶处理导致结合活性有不同程度的增加。结合的增加是由于结合亲和力和/或结合位点浓度的增加。蛋白质修饰试剂乙酸酐、N-乙基马来酰亚胺和巯基乙醇均降低了结合。这些结果表明膜的蛋白质、脂质和碳水化合物成分在PGE2与其结合位点之间的结合相互作用中具有重要性。巯基乙醇和N-乙基马来酰亚胺降低结合的能力表明巯基和二硫键均参与了PGE2结合反应。
