Department of Macromolecular Science and Engineering, Graduate School of Science & Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
Department of Macromolecular Science and Engineering, Graduate School of Science & Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
Ultrasonics. 2022 Feb;119:106581. doi: 10.1016/j.ultras.2021.106581. Epub 2021 Sep 25.
The latex agglutination test is employed to visualize antigen-antibody reactions through the aggregation of antibody-coated particles in the presence of an antigen. In the present study, we developed an ultrasound scattering technique to detect latex agglutination in an optically turbid media. However, the ultrasonic technique had less sensitivity to the dilute particle suspension than the optical techniques because of its wavelength. Therefore, we applied a time-correlation approach to detect small amounts of these aggregates using a sophisticated noise correction algorithm in the frequency domain. The lowest concentration of avidin used to detect aggregations of the biotin-coated particle using the ultrasound scattering technique was found to be 0.625 μg/ml. Furthermore, since the density differences between the particle and liquid were larger for silica suspensions than for polystyrene (PS) suspensions, a larger signal was proposed to be expected from silica suspensions. Nevertheless, it was found that latex agglutinations with the PS particle were more sensitive than those with the silica particles. The dynamic ultrasound scattering analysis along the sedimentation direction also supported the presence of strongly scattered intensity components of the PS aggregates, which is proposed to be due to the resonance scattering from PS spherical particles. Therefore, this technique can be employed to enhance scattering signals from particles for application in the agglutination test using ultrasound.
胶乳凝集试验用于通过在存在抗原的情况下使抗体包被的颗粒聚集来可视化抗原-抗体反应。在本研究中，我们开发了一种超声散射技术来检测光学混浊介质中的胶乳凝集。然而，由于其波长，超声技术对稀释颗粒悬浮液的灵敏度低于光学技术。因此，我们应用了一种时间相关方法，通过在频域中使用复杂的噪声校正算法来检测这些小量聚集物。使用超声散射技术检测生物素包被颗粒聚集的最低浓度的亲和素被发现为 0.625μg/ml。此外，由于硅胶悬浮液的颗粒和液体之间的密度差异大于聚苯乙烯 (PS) 悬浮液，因此预计硅胶悬浮液会产生更大的信号。然而，发现 PS 颗粒的胶乳凝集比硅胶颗粒更敏感。沿沉降方向的动态超声散射分析也支持 PS 聚集体存在强烈散射强度分量，这归因于 PS 球形颗粒的共振散射。因此，该技术可用于增强用于超声凝集试验的颗粒的散射信号。
