Department of Traditional Chinese Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China; Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing 100045, China.
Department of Traditional Chinese Medicine, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.
J Affect Disord. 2022 Jan 1;296:150-156. doi: 10.1016/j.jad.2021.09.064. Epub 2021 Sep 26.
The relationship between depression and adaptive immunity has gradually attracted increasing attentions. However, no consistent conclusions have been drawn about alterations in adaptive immunity in depression.
Eight-week-old weight-matched male C57BL/6 mice were randomly divided into a chronic unpredictable mild stress (CUMS) model group and a control group, with twelve mice in each group. After 8-week CUMS modeling duration and depressive behavioral tests, T cell responses were evaluated at the cell, cytokine, and cell-specific transcription factor levels. Lymphocytes in the spleen were detected by flow cytometry; serum cytokines were assessed by enzyme-linked immunosorbent assays; expression levels of cytokines and cell-specific transcription factors in the hippocampus were determined by quantitative PCR.
CUMS exposure induced depression-like behaviors with decreased sucrose preference and longer immobility time in the tail suspension test and forced swim test. The CUMS group had a lower proportion of CD4T cells than the control group. A decreased number of T helper 17 (Th17) cells in the spleen and down regulated serum interleukin (IL)-17 and hippocampal retinoid-related orphan receptor-γt levels were detected in the CUMS group. Depressed mice in the CUMS group showed increases in serum IL-1β and IL-6 and hippocampal IL-6 and IL-4 levels.
The study was based on a mouse model. Further studies are needed to determine whether the results are applicable to patients with depression.
CUMS exposure can induce depression-like behaviors and decrease Th17 cell counts.
抑郁症与适应性免疫之间的关系逐渐引起了越来越多的关注。然而，关于抑郁症患者适应性免疫的改变，尚未得出一致的结论。
将 8 周龄体重匹配的雄性 C57BL/6 小鼠随机分为慢性不可预测轻度应激（CUMS）模型组和对照组，每组 12 只。经过 8 周 CUMS 造模时间和抑郁行为测试后，在细胞、细胞因子和细胞特异性转录因子水平评估 T 细胞反应。采用流式细胞术检测脾淋巴细胞；采用酶联免疫吸附试验检测血清细胞因子；采用定量 PCR 检测海马细胞因子和细胞特异性转录因子的表达水平。
CUMS 暴露导致抑郁样行为，在悬尾试验和强迫游泳试验中，糖水偏好度降低，不动时间延长。与对照组相比，CUMS 组 CD4T 细胞比例较低。CUMS 组脾内 Th17 细胞数量减少，血清白细胞介素（IL）-17 和海马维甲酸相关孤儿受体-γt 水平降低。与对照组相比，CUMS 组血清 IL-1β 和 IL-6 及海马 IL-6 和 IL-4 水平升高。
该研究基于小鼠模型。需要进一步的研究来确定这些结果是否适用于抑郁症患者。
CUMS 暴露可引起抑郁样行为，并减少 Th17 细胞计数。
