Laboratory of Research on Biologically Compatible Compounds, Faculty of Dentistry, University of Monastir, Street Avicenne, 5000, Monastir, Tunisia.
Department of Nephrology, Dialysis, and Transplant, University Hospital of Sahloul, Sousse, Tunisia.
Environ Sci Pollut Res Int. 2022 Feb;29(10):14111-14120. doi: 10.1007/s11356-021-16091-8. Epub 2021 Oct 3.
Bromuconazole is a triazole pesticide used to protect vegetables and fruits against diverse fungi pathologies. However, its utilization may be accompanied by diverse tissue injuries. In this study, we evaluated the biochemical and histopathological modifications, and we analyzed genotoxic and oxidative stress, in the aim to examine bromuconazole effects in the liver and kidney. We subdivided animals into four groups, each one contains six adult male Wistar rats. Untreated rats received daily a corn oil (vehicle) orally. Three oral bromuconazole doses were tested (1, 5, and 10 % of LD50) daily for 28 days. Bromuconazole increased the plasma activities of alkaline phosphatase, lactate dehydrogenase, and transaminases. It also increased the plasma levels of creatinine and uric acid. Histopathological check showed that bromuconazole caused organ damage. This study makes known that bromuconazole caused conspicuous DNA damage either in hepatic or kidney tissues, with a significant increase in the levels of malondialdehyde and protein carbonyl followed by an enhancement in catalase and superoxide dismutase enzymatic activities, and these increases are in a dose-dependent manner. In other side, we found that Glutathione-S-transferase and peroxidase activities raised. Our outcomes highlight that bromuconazole exposure induced genotoxic damage and organ damage which may be caused by the disturbances of oxidative stress statue in the liver and kidney.
溴菌腈是一种三唑类农药，用于保护蔬菜和水果免受多种真菌病害的侵害。然而，它的使用可能伴随着各种组织损伤。在这项研究中，我们评估了生化和组织病理学的变化，并分析了遗传毒性和氧化应激，旨在检查溴菌腈对肝脏和肾脏的影响。我们将动物分为四组，每组包含六只成年雄性 Wistar 大鼠。未处理的大鼠每天口服玉米油（载体）。测试了三种口服溴菌腈剂量（LD50 的 1%、5%和 10%），每天一次，共 28 天。溴菌腈增加了碱性磷酸酶、乳酸脱氢酶和转氨酶的血浆活性。它还增加了血浆中肌酐和尿酸的水平。组织病理学检查显示，溴菌腈导致器官损伤。本研究表明，溴菌腈在肝或肾组织中引起明显的 DNA 损伤，伴随着丙二醛和蛋白质羰基水平的显著增加，随后过氧化氢酶和超氧化物歧化酶的酶活性增强，这些增加呈剂量依赖性。另一方面，我们发现谷胱甘肽-S-转移酶和过氧化物酶的活性升高。我们的结果表明，溴菌腈暴露引起的遗传毒性损伤和器官损伤可能是由于肝脏和肾脏氧化应激状态的紊乱所致。
