Lu Ziyun, Chen Meiling, Long Xinyi, Yang Huilin, Zhu Du
Key Laboratory of Protection and Utilization of Subtropic Plant Resources of Jiangxi Province, Jiangxi Normal University, Nanchang, China.
Key Laboratory of Bioprocess Engineering of Jiangxi Province, Jiangxi Science and Technology Normal University, Nanchang, China.
Front Microbiol. 2023 Feb 20;14:1064838. doi: 10.3389/fmicb.2023.1064838. eCollection 2023.
Fusarium root rot (FRR) caused by poses a threat to global food security. Biological control is a promising control strategy for FRR. In this study, antagonistic bacteria were obtained using an dual culture bioassay with . Molecular identification of the bacteria based on the 16S rDNA gene and whole genome revealed that the species belonged to the genus . We evaluated the strain BS45 for its mechanism against phytopathogenic fungi and its biocontrol potential against FRR caused by . A methanol extract of BS45 caused swelling of the hyphal cells and the inhibition of conidial germination. The cell membrane was damaged and the macromolecular material leaked out of cells. In addition, the mycelial reactive oxygen species level increased, mitochondrial membrane potential decreased, oxidative stress-related gene expression level increased and oxygen-scavenging enzyme activity changed. In conclusion, the methanol extract of BS45 induced hyphal cell death through oxidative damage. A transcriptome analysis showed that differentially expressed genes were significantly enriched in ribosome function and various amino acid transport pathways, and the protein contents in cells were affected by the methanol extract of BS45, indicating that it interfered with mycelial protein synthesis. In terms of biocontrol capacity, the biomass of wheat seedlings treated with the bacteria increased, and the BS45 strain significantly inhibited the incidence of FRR disease in greenhouse tests. Therefore, strain BS45 and its metabolites are promising candidates for the biological control of and its related root rot diseases.
由镰刀菌引起的根腐病对全球粮食安全构成威胁。生物防治是一种有前景的根腐病防治策略。在本研究中，通过与[具体对象]进行对峙培养生物测定获得了拮抗细菌。基于16S rDNA基因和全基因组对细菌进行分子鉴定，结果表明该菌种属于[具体属名]属。我们评估了BS45菌株对植物病原真菌的作用机制及其对由[具体病原]引起的根腐病的生防潜力。BS45的甲醇提取物导致菌丝细胞肿胀并抑制分生孢子萌发。细胞膜受损，大分子物质从细胞中泄漏出来。此外，菌丝活性氧水平升高，线粒体膜电位降低，氧化应激相关基因表达水平升高，抗氧化酶活性发生变化。总之，BS45的甲醇提取物通过氧化损伤诱导菌丝细胞死亡。转录组分析表明，差异表达基因在核糖体功能和各种氨基酸转运途径中显著富集，细胞中的蛋白质含量受到BS45甲醇提取物的影响，这表明它干扰了菌丝体蛋白质合成。在生防能力方面，用该细菌处理的小麦幼苗生物量增加，并且在温室试验中BS45菌株显著抑制了根腐病的发病率。因此，BS45菌株及其代谢产物是防治[具体病原]及其相关根腐病的有前景的候选物。
