Beelagi Mallikarjun S, Kumar Sr Santosh, Indrabalan Uma Bharathi, Patil Sharanagouda S, Prasad Ashwini, Suresh K P, Kollur Shiva Prasad, Jayappa Veeresh Santhebennur, Kakkalameli Siddappa B, Srinivasa Chandrashekar, Venkataravana Prabhakarareddy Anapalli, Shivamallu Chandan
Department of Biotechnology and Bioinformatics, Faculty of Life Sciences, JSS Academy of Higher Education & Research, Mysuru-570015, India.
Department of Studies in Food Technology, Shivagangotri, Davangere University, Davangere Karnataka-577 007, India.
Bioinformation. 2021 Apr 30;17(4):479-491. doi: 10.6026/97320630017479. eCollection 2021.
Crimean-Congo hemorrhagic fever (CCHF) virus is one among the major zoonosis viral diseases that use the Hyalomma ticks as their transmission vector to cause viral infection to the human and mammalian community. The fatality of infectious is high across the world especially in Africa, Asia, Middle East, and Europe. This study regarding codon usage bias of S, M, and L segments of the CCHF virus pertaining to the host Homo sapiens, reveals in-depth information about the evolutionary characteristics of CCHFV. Relative Synonymous Codon Usage (RSCU), Effective number of codons (ENC) were calculated, to determine the codon usage pattern in each segment. Correlation analysis between Codon adaptation index (CAI), GRAVY (Hydrophobicity), AROMO (Aromaticity), and nucleotide composition revealed bias in the codon usage pattern. There was no strong codon bias found among any segments of the CCHF virus, indicating both the factors i.e., natural selection and mutational pressure shapes the codon usage bias.
克里米亚-刚果出血热（CCHF）病毒是主要的人畜共患病毒性疾病之一，它以璃眼蜱作为传播媒介，可导致人类和哺乳动物群体发生病毒感染。在全球范围内，尤其是在非洲、亚洲、中东和欧洲，该传染病的致死率很高。这项关于CCHF病毒S、M和L片段相对于宿主智人的密码子使用偏好的研究，揭示了有关CCHFV进化特征的深入信息。计算了相对同义密码子使用情况（RSCU）、有效密码子数（ENC），以确定每个片段中的密码子使用模式。密码子适应指数（CAI）、亲水性（GRAVY）、芳香性（AROMO）与核苷酸组成之间的相关性分析揭示了密码子使用模式存在偏好。在CCHF病毒的任何片段中均未发现强烈的密码子偏好，这表明自然选择和突变压力这两个因素共同塑造了密码子使用偏好。
