对……中转录组数据库的同义密码子使用情况进行分析。 （你提供的原文似乎不完整，最后的“in.”后面应该还有具体内容）
Analysis of synonymous codon usage of transcriptome database in .
作者信息
Huo Xiaowei, Liu Sisi, Li Yimin, Wei Hao, Gao Jing, Yan Yonggang, Zhang Gang, Liu Mengmeng
机构信息
College of Pharmaceutical Science, Institute of Life Science and Green Development, Hebei University, Baoding, China.
Hunan Academy of Forestry, Changsha, China.
出版信息
PeerJ. 2021 Jan 4;9:e10450. doi: 10.7717/peerj.10450. eCollection 2021.
BACKGROUND
is an endangered and important medicinal plant in Asian countries, especially in China. However, there is little knowledge about the codon usage bias for CDSs. In this project, codon usage bias was determined based on the 2,626 predicted CDSs from R. palmatum transcriptome.
METHODS
In this study, all codon usage bias parameters and nucleotide compositions were calculated by Python script, Codon W, DNA Star, CUSP of EMBOSS.
RESULTS
The average GC and GC3 content are 46.57% and 46.6%, respectively, the results suggested that there exists a little more AT than GC in the genes, and the codon bias of genes preferred to end with A/T. We concluded that the codon bias in was affect by nucleotide composition, mutation pressure, natural selection, gene expression levels, and the mutation pressure is the prominent factor. In addition, we figured out 28 optimal codons and most of them ended with A or U. The project here can offer important information for further studies on enhancing the gene expression using codon optimization in heterogeneous expression system, predicting the genetic and evolutionary mechanisms in .
背景
是亚洲国家尤其是中国一种濒危且重要的药用植物。然而，关于其编码序列（CDS）的密码子使用偏好却知之甚少。在本项目中，基于掌叶大黄转录组预测的2626个CDS确定了密码子使用偏好。
方法
在本研究中，所有密码子使用偏好参数和核苷酸组成均通过Python脚本、Codon W、DNA Star、EMBOSS的CUSP进行计算。
结果
平均GC含量和GC3含量分别为46.57%和46.6%，结果表明该植物基因中AT含量略多于GC，且基因的密码子偏好以A/T结尾。我们得出结论，掌叶大黄的密码子偏好受核苷酸组成、突变压力、自然选择、基因表达水平影响，且突变压力是主要因素。此外，我们确定了28个最优密码子，其中大多数以A或U结尾。本项目可为进一步研究在异源表达系统中利用密码子优化提高基因表达、预测掌叶大黄的遗传和进化机制提供重要信息。
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