College of Bioscience and Biotechnology, Hunan Agricultural University, Hunan Provincial Engineering Research Center of Applied Microbial Resources Development for Livestock and Poultry, Changsha, Hunan 410125, China.
Mediators Inflamm. 2021 Sep 23;2021:2752265. doi: 10.1155/2021/2752265. eCollection 2021.
The mice were randomly distributed into four groups: (a) control (CTRL) group, (b) ETEC group, (c) IQW-ETEC group, and (d) IRW-ETEC group. Villus length and crypt depth were measured after hematoxylin and eosin staining. The inflammatory reaction was analyzed via inflammatory cytokines (i.e., TNF-, IL-1, IL-6, and IL-10) using the enzyme-linked immunosorbent assay (ELISA). The microbiota in the colon was sequenced using 16S ribosomal RNA.
The villus length decreased, the crypt depth decreased, and the expression of inflammatory cytokines (i.e., TNF-, IL-1, IL-6, and IL-10) increased due to ETEC. In the IRW-ETEC and IQW-ETEC groups, the Shannon index decreased ( < 0.05). IQW and IRW increased the abundance of , , , , and ; contrastingly, it decreased the abundance of , , , and compared to the ETEC group ( <0.05).
This study ascertained that the addition of IQW and IRW could alleviate jejunal inflammation and increase microbiota community diversity.
将小鼠随机分为四组：（a）对照组（CTRL）、（b）肠致病性大肠杆菌（ETEC）组、（c）IQW-ETEC 组和（d）IRW-ETEC 组。经苏木精-伊红（H&E）染色后测量绒毛长度和隐窝深度。采用酶联免疫吸附试验（ELISA）分析炎性细胞因子（即 TNF-α、IL-1、IL-6 和 IL-10）的炎症反应。通过 16S 核糖体 RNA 对结肠中的微生物群进行测序。
由于 ETEC，绒毛长度降低，隐窝深度降低，炎性细胞因子（即 TNF-α、IL-1、IL-6 和 IL-10）的表达增加。在 IRW-ETEC 和 IQW-ETEC 组中，香农指数降低（<0.05）。与 ETEC 组相比，IQW 和 IRW 增加了 、 、 、 和 的丰度；相反，它降低了 、 、 和 的丰度（<0.05）。
本研究证实，添加 IQW 和 IRW 可减轻空肠炎症并增加微生物群落多样性。
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