Hilgers J, van der Sluis P J, van Blitterswijk W J, Emmelot P
Br J Cancer. 1978 Mar;37(3):329-36. doi: 10.1038/bjc.1978.51.
Transplantation of primary GRSL cells in the ascitic form led to a decrease in membrane microviscosity as measured by the fluorescence polarization technique. The transplanted GRSL ascitic cells showed a markedly lower ability to form caps with respect to both virus-related (MLr, GIX) and normal (H-2.7(G), H-2.8(K) and TL1.2) cell-surface antigens and their appropriate antisera in the indirect membrane immunofluorescence tests, than did primary GRSL cells, transplanted GRSL cells growing in solid form, and thymocytes, which all exhibited significantly higher membrane microviscosities. Transplantation of primary GRSL cells into syngeneic mice pre-irradiated with 400 rad did not lead to a fall in membrane microviscosity. It is suggested that the host immune response in intact mice leads to a selective survival of ascitic tumour cells with low membrane microviscosity.
以腹水形式移植原代GRSL细胞导致通过荧光偏振技术测量的膜微粘度降低。在间接膜免疫荧光试验中，与病毒相关（MLr、GIX）和正常（H-2.7(G)、H-2.8(K)和TL1.2）细胞表面抗原及其相应抗血清相比，移植的GRSL腹水细胞形成帽的能力明显较低，而原代GRSL细胞、以固体形式生长的移植GRSL细胞和胸腺细胞形成帽的能力明显较高，它们都表现出明显更高的膜微粘度。将原代GRSL细胞移植到预先接受400拉德照射的同基因小鼠中不会导致膜微粘度下降。提示完整小鼠中的宿主免疫反应导致具有低膜微粘度的腹水肿瘤细胞选择性存活。
