Hilgers J, van Blitterswijk W J, Bont W S, Theuns G J, Nusse R, Haverman J, Emmelot P
J Natl Cancer Inst. 1975 Jun;54(6):1335-42. doi: 10.1093/jnci/54.6.1335.
By means of the indirect membrane immunofluorescence test, the distribution and antibody-induced redistribution (patching and capping) of a mammary tumor virus-induced (MLr) and a normal (Thy 1.2) cell-surface antigen were compared on mouse thymocytes and leukemia cells (GRSL2). At 0 degrees C Thy 1.2 fluorescence was ringlike and more intense on GRSL2 cells than on thymocytes, whereas MLr fluorescence on GRLS2 cells at this temperature was patchlike and brighter than Thy 1.2 fluorescence. At 20 or 37 degrees C, capping of Thy 1.2 on both cell types was readily achieved but MLr capping occurred only in a few GRSL2 cells and was less pronounced. However, after addition of the secondary antibodies, MLr capping was markedly increased by gradual cooling of cells to about 17 degrees C. Conversely, after addition of antibodies at 0 degrees C, gradual warming of cells under the fluorescence microscope resulted in extensive capping both of MLr and Thy 1.2 at approximately 13-14 degrees C. Rapid cooling or rapid warming led to almost instantaneous capping. These results may be explained by the occurrence of phase transitions or phase separations in the particular temperature range. Another difference between capping of Thy 1.2 and MLr was that the former caps were small and eventually were endocytosed, whereas the MLr caps were large and were exfoliated from the cells.
通过间接膜免疫荧光试验，比较了乳腺肿瘤病毒诱导的（MLr）和正常的（Thy 1.2）细胞表面抗原在小鼠胸腺细胞和白血病细胞（GRSL2）上的分布以及抗体诱导的再分布（成斑和盖帽）情况。在0℃时，Thy 1.2荧光呈环状，在GRSL2细胞上比在胸腺细胞上更强烈，而此时GRLS2细胞上的MLr荧光呈斑块状，且比Thy 1.2荧光更亮。在20或37℃时，两种细胞类型上的Thy 1.2都很容易形成盖帽，但MLr盖帽仅在少数GRSL2细胞中出现，且不太明显。然而，加入二抗后，通过将细胞逐渐冷却至约17℃，MLr盖帽明显增加。相反，在0℃加入抗体后，在荧光显微镜下逐渐升温细胞会导致在约13 - 14℃时MLr和Thy 1.2都广泛形成盖帽。快速冷却或快速升温会导致几乎瞬间形成盖帽。这些结果可能是由于在特定温度范围内发生了相变或相分离。Thy 1.2和MLr盖帽之间的另一个区别是，前者的盖帽小，最终被内吞，而MLr的盖帽大，并从细胞上脱落。
