The present study aimed to report a novel electrochemical sensor through electropolymerization of -aminophenol (-AP) and -dihydroxy benzene (-DB) as monomers on the surface of the glassy carbon electrode (GCE) for the determination of histidine (His) as a template molecule. The developed sensor exhibited satisfactory sensitivity and high selectivity, and also offered a linear range between 0.005 and 10.0 μM with a detection limit of 0.9 nM. Finally, it is worth mentioning that we also aimed at employing the proposed sensor for the detection of His in blood serum samples.