Chasta Himanshu, Goyal Rajendra N
Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India.
Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India.
Talanta. 2014 Jul;125:167-73. doi: 10.1016/j.talanta.2014.02.038. Epub 2014 Mar 10.
An electrochemical sensor has been developed for the selective determination of norepinephrine (NE) using the molecularly imprinted technique. The imprinted polymer film at the surface of glassy carbon electrode is prepared by the electropolymerization of o-aminophenol in the presence of NE. Imprinted polymer film was characterized by atomic force microscopy (AFM), field emission scanning electron microscopy (FE-SEM), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The imprinted sensor showed a well-defined anodic peak at a potential of ~198 mV in phosphate buffer of pH 7.2 using square wave voltammetry. A linear increase in peak current was found with the increasing concentration of NE in the range from 50×10(-9) to 10×10(-6)mol L(-1)and the limit of detection (3σ/b) was found to be 4.9×10(-10)mol L(-1). The imprinted sensor has been successfully employed to ascertain the content of NE in the commercially available pharmaceutical preparations. The biological applicability of the developed sensor has been delineated by the determination of NE in human plasma and urine samples using the standard addition method. The proposed sensor exhibited high degree of selectivity for NE in comparison to other structurally similar biomolecules present in biological samples, along with long term stability, good reproducibility and excellent capacity of regeneration of molecular recognition sites.
已开发出一种电化学传感器，采用分子印迹技术选择性测定去甲肾上腺素（NE）。在玻碳电极表面的印迹聚合物膜是通过邻氨基酚在NE存在下的电聚合制备的。通过原子力显微镜（AFM）、场发射扫描电子显微镜（FE-SEM）、电化学阻抗谱（EIS）和循环伏安法（CV）对印迹聚合物膜进行了表征。使用方波伏安法时，印迹传感器在pH 7.2的磷酸盐缓冲液中，在约198 mV的电位处显示出明确的阳极峰。随着NE浓度在50×10⁻⁹至10×10⁻⁶ mol L⁻¹范围内增加，峰电流呈线性增加，检测限（3σ/b）为4.9×10⁻¹⁰ mol L⁻¹。印迹传感器已成功用于确定市售药物制剂中NE的含量。通过使用标准加入法测定人血浆和尿液样本中的NE，描述了所开发传感器的生物适用性。与生物样品中存在的其他结构相似的生物分子相比，所提出的传感器对NE表现出高度的选择性，同时具有长期稳定性、良好的重现性和分子识别位点的优异再生能力。
