Department of Morphology, Institute of Anatomy and Anthropology, Riga Stradins University, Riga, Latvia.
Folia Morphol (Warsz). 2022;81(3):584-593. doi: 10.5603/FM.a2021.0084. Epub 2021 Oct 5.
The aim of this study was to evaluate morphologic features of healthy saphenous vein and internal thoracic artery, blood vessels used in coronary artery bypass graft (CABG) surgery, and compare results.
Ten specimens of saphenous veins and ten of internal thoracic arteries used for CABG were obtained from 20 patients. Histological routine and immunohistochemical staining was performed with: endothelin (ET), tissue inhibitor of metalloproteinase 2 (TIMP2), metallomembranoproteinase 2 (MMP2), transforming growth factor beta (TGFβ), hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), protein gene product 9.5 (PGP9.5), vascular cell adhesion molecule (VCAM), intercellular adhesion molecule (ICAM). A semiquantitative evaluation method was used.
There was found: a moderate number of endothelin-positive cells in both blood vessel types; a moderate number of MMP2-positive cells and moderate in number to numerous TIMP2-positive cells in veins. In arteries - occasionally marked positive MMP2 cells and negative TIMP2; moderate in number to numerous VEGF-positive endothelial cells on small blood vessels in vein wall and occasionally in artery wall; numerous TGFβ-positive structures in veins and abundance of VCAM- and ICAM-positive cells, few in arteries; few HGF-positive structures in veins, negative in arteries; In veins, few PGP9.5-positive nerve fibres, in arteries - moderate. Moderate TUNEL reaction-positive apoptotic cells in veins and few to moderate in arteries.
Vena saphena magna grafts are characterised by increased plasticity when it comes to modelling. Number of VEGF, VCAM and ICAM found in vena saphena magna proves the possible tendency of graft failure on basis of local blood supply intensification. Appearance of endothelin positive cells indicate the similar homeostasis condition in endotheliocytes in both - vein and artery grafts.
本研究旨在评估用于冠状动脉旁路移植术（CABG）的健康大隐静脉和内乳动脉的形态特征，并比较结果。
从 20 名患者中获得了 10 个大隐静脉和 10 个内乳动脉标本，用于 CABG。进行了组织学常规和免疫组织化学染色，使用：内皮素（ET）、金属基质蛋白酶 2 组织抑制剂（TIMP2）、金属基质蛋白酶 2（MMP2）、转化生长因子-β（TGFβ）、肝细胞生长因子（HGF）、血管内皮生长因子（VEGF）、蛋白基因产物 9.5（PGP9.5）、血管细胞黏附分子（VCAM）、细胞间黏附分子（ICAM）。使用半定量评估方法。
发现：两种血管类型中均有中等数量的内皮素阳性细胞；静脉中中等数量至大量 MMP2 阳性细胞和中等数量至大量 TIMP2 阳性细胞。在动脉中-偶尔有明显的 MMP2 阳性细胞和 TIMP2 阴性；静脉壁小血管内皮细胞中存在中等数量至大量 VEGF 阳性细胞，偶尔在动脉壁中也有；静脉中存在大量 TGFβ 阳性结构和丰富的 VCAM 和 ICAM 阳性细胞，动脉中较少；静脉中存在少量 HGF 阳性结构，动脉中阴性；静脉中有少量 PGP9.5 阳性神经纤维，动脉中中等数量。静脉中存在中等数量至大量 TUNEL 反应阳性凋亡细胞，动脉中较少。
大隐静脉移植物具有较高的可塑性，当涉及到建模时。大隐静脉中发现的 VEGF、VCAM 和 ICAM 数量表明，基于局部血液供应的增强，移植物可能存在失败的趋势。内皮素阳性细胞的出现表明，两种血管-静脉和动脉移植物的内皮细胞具有相似的内稳态条件。
