Mukherjee Tarushyam, Regar Ramprasad, Soppina Virupakshi, Kanvah Sriram
Discipline of Chemistry, Indian Institute of Technology Gandhinagar, Palaj, Gandhinagar 382355, India.
Discipline of Biological Engineering, Indian Institute of Technology Gandhinagar, Palaj, Gandhinagar 382355, India.
Org Biomol Chem. 2021 Dec 1;19(46):10090-10096. doi: 10.1039/d1ob01741a.
The 'powerhouses' of cell, mitochondria have seen an upsurge of interest in investigations pertaining to the imaging and mapping of physiological processes. By utilizing sterol-modified rhodamine, we have performed the live-cell imaging of mitochondria without dependence on a membrane potential. The sterol probes are highly biocompatible, and they can track the mitochondrial live-cell dynamics in a background-free manner with improved brightness and impressive contrast. This is the first attempt to study the stress response using a direct fluorescence readout with bio-conjugates of rhodamine inside mitochondria. The results pave the way for developing different sterol markers for understanding cellular responses and function.
细胞的“动力源”——线粒体，在与生理过程成像和图谱绘制相关的研究中受到了越来越多的关注。通过使用甾醇修饰的罗丹明，我们实现了不依赖膜电位的线粒体活细胞成像。甾醇探针具有高度的生物相容性，它们能够以无背景的方式追踪线粒体活细胞动态，亮度更高，对比度令人印象深刻。这是首次尝试使用直接荧光读数，通过线粒体内罗丹明的生物偶联物来研究应激反应。这些结果为开发不同的甾醇标记物以理解细胞反应和功能铺平了道路。
