Kamdhenu University, Gandhinagar, Gujarat, 382010, India.
Trop Anim Health Prod. 2021 Oct 6;53(5):501. doi: 10.1007/s11250-021-02955-1.
The objective of the study was to evaluate the diagnostic performance of the electronic handheld BHBCheck meter (BHM) (PortaCheck, Inc., USA) to determine whole blood, plasma and serum β-hydroxybutyrate (BHB) against serum BHB determined using reference laboratory method of Randox D-3 Hydroxybutyrate Ranbut assay (RSM) in Indian dairy cows and buffaloes. Blood samples were collected by puncturing coccygeal vessels for determining whole blood, serum and plasma BHB using BHM and serum BHB using RSM from 217 cows (Gir breed; median 42 DIM and 3rd lactation) and 223 buffaloes (non-descript; median 39 DIM and 3rd lactation) from nearby herds. The Pearson's correlation between whole blood (0.988; 0.987), plasma (0.985; 0.983) and serum (0.985; 0.983) BHB determined using the BHM and serum BHB determined with the RSM in Indian cows and buffaloes, respectively, were significant. Bland-Altman plot demonstrated an excellent agreement between whole blood, plasma and serum BHB determined with BHM, against the serum BHB determined with RSM in Indian cows and buffaloes, respectively. For hyperketonaemia with reference serum BHB cut-off values ≥ 1.2 and 1.4 mmol/L determined with RSM, it recorded optimized BHB thresholds, sensitivity and specificity for whole blood (≥ 0.9 to 1.0 mmol/L; 91 to 95% and 88 to 98%), plasma (≥ 0.9 to 1.0 mmol/L; 91 and 100%) and serum (≥ 0.9 to 1.0 mmol/L; 92 to 100% and 85 to 94%) with BHM in cows and buffaloes, respectively. In conclusion, BHB determined with BHM demonstrated an excellent correlation, agreement and test characteristics with BHB determined with RSM and hence can accurately determine whole blood, plasma and serum BHB in cows and buffaloes.
本研究旨在评估电子 handheld BHBCheck meter（BHM）（PortaCheck，Inc.，美国）用于测定全血、血浆和血清 β-羟丁酸（BHB）的诊断性能，方法是将其与使用参考实验室方法 Randox D-3 Hydroxybutyrate Ranbut 测定法（RSM）测定的血清 BHB 进行比较，该方法用于测定印度奶牛和水牛的血清 BHB。通过刺破尾骨血管采集血液样本，使用 BHM 测定全血、血清和血浆 BHB，使用 RSM 测定血清 BHB，共采集了 217 头奶牛（Gir 品种；中位数为 42 天产犊，第 3 次产犊）和 223 头水牛（非特定品种；中位数为 39 天产犊，第 3 次产犊）的血液样本。印度奶牛和水牛的全血（0.988；0.987）、血浆（0.985；0.983）和血清（0.985；0.983）BHB 用 BHM 测定与 RSM 测定的血清 BHB 之间的 Pearson 相关性均显著。Bland-Altman 图显示，BHM 测定的全血、血浆和血清 BHB 与 RSM 测定的血清 BHB 在印度奶牛和水牛中具有极好的一致性。对于参考血清 BHB 截断值为≥1.2 和 1.4 mmol/L 的高酮血症，BHM 记录了优化的 BHB 阈值、敏感性和特异性，用于全血（≥0.9 至 1.0 mmol/L；91%至 95%和 88%至 98%）、血浆（≥0.9 至 1.0 mmol/L；91%和 100%）和血清（≥0.9 至 1.0 mmol/L；92%至 100%和 85%至 94%）。总之，BHM 测定的 BHB 与 RSM 测定的 BHB 具有极好的相关性、一致性和检测特征，因此可以准确地测定奶牛和水牛的全血、血浆和血清 BHB。
