Henan Institute of Crop Molecular Breeding, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.
Agronomy College, Zhengzhou University, 450001, Zhengzhou, China.
BMC Plant Biol. 2021 Oct 6;21(1):455. doi: 10.1186/s12870-021-03221-0.
Glutenin contents and compositions are crucial factors influencing the end-use quality of wheat. Although the composition of glutenin fractions is well known, there has been relatively little research on the genetic basis of glutenin fractions in wheat.
To elucidate the genetic basis for the contents of glutenin and its fractions, a population comprising 196 recombinant inbred lines (RILs) was constructed from two parents, Luozhen No.1 and Zhengyumai 9987, which differ regarding their total glutenin and its fraction contents (except for the By fraction). Forty-one additive Quantitative Trait Loci (QTL) were detected in four environments over two years. These QTL explained 1.3% - 53.4% of the phenotypic variation in the examined traits. Forty-three pairs of epistatic QTL (E-QTL) were detected in the RIL population across four environments. The QTL controlling the content of total glutenin and its seven fractions were detected in clusters. Seven clusters enriched with QTL for more than three traits were identified, including a QTL cluster 6AS-3, which was revealed as a novel genetic locus for glutenin and related traits. Kompetitive Allele-Specific PCR (KASP) markers developed from the main QTL cluster 1DL-2 and the previously developed KASP marker for the QTL cluster 6AS-3 were validated as significantly associated with the target traits in the RIL population and in natural varieties.
This study identified novel genetic loci related to glutenin and its seven fractions. Additionally, the developed KASP markers may be useful for the marker-assisted selection of varieties with high glutenin fraction content and for identifying individuals in the early developmental stages without the need for phenotyping mature plants. On the basis of the results of this study and the KASP markers described herein, breeders will be able to efficiently select wheat lines with favorable glutenin properties and develop elite lines with high glutenin subunit contents.
谷蛋白含量和组成是影响小麦食用品质的关键因素。尽管谷蛋白组分的组成已经众所周知，但关于小麦谷蛋白组分的遗传基础的研究相对较少。
为了阐明谷蛋白及其组分含量的遗传基础，从两个亲本品种（洛夫林 1 号和郑麦 9987）构建了一个包含 196 个重组自交系（RIL）的群体，它们在总谷蛋白及其组分（除 By 组分外）含量方面存在差异。在两年的四个环境中检测到 41 个加性数量性状位点（QTL）。这些 QTL 解释了所研究性状表型变异的 1.3%至 53.4%。在四个环境中，在 RIL 群体中检测到 43 对上位性 QTL（E-QTL）。控制总谷蛋白及其七个组分含量的 QTL 聚集在簇中。鉴定出 7 个富含三个以上性状 QTL 的簇，包括一个 QTL 簇 6AS-3，该簇被揭示为谷蛋白和相关性状的新遗传位点。从主要 QTL 簇 1DL-2 和先前开发的 QTL 簇 6AS-3 的 KASP 标记开发的 Kompetitive Allele-Specific PCR (KASP) 标记在 RIL 群体和自然品种中验证与目标性状显著相关。
本研究鉴定了与谷蛋白及其七个组分相关的新遗传位点。此外，开发的 KASP 标记可用于高谷蛋白组分含量品种的标记辅助选择，以及在无需表型成熟植株的情况下鉴定早期发育阶段的个体。基于本研究的结果和本文描述的 KASP 标记，育种家将能够有效地选择具有有利谷蛋白特性的小麦品系，并开发具有高谷蛋白亚基含量的优秀品系。
