Wheat Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China.
College of Chemistry and Environment Engineering, Pingdingshan University, Pingdingshan 467000, China.
Int J Mol Sci. 2020 Mar 11;21(6):1928. doi: 10.3390/ijms21061928.
Micronutrient deficiencies, and especially zinc (Zn) deficiency, pose serious health problems to people who mainly depend on cereal-based diets. Here, we performed a genome-wide association study (GWAS) to detect the genetic basis of the Zn accumulation in wheat ( L.) grains with a diversity panel of 207 bread wheat varieties. To uncover authentic quantitative trait loci (QTL) controlling Zn accumulation, the varieties were planted in three locations. In total, 29 unique loci associated with Zn grain accumulation were identified. Notably, seven non-redundant loci located on chromosomes 1B, 3B, 3D, 4A, 5A, 5B, and 7A, were detected at least in two environments. Of these quantitative trait loci (QTL), six coincided with known QTL or genes, whereas the highest effect QTL on chromosome 3D identified in this study was not reported previously. Searches of public databases revealed that the seven identified QTL coincided with seven putative candidate genes linked to Zn accumulation. Among these seven genes, gene () linked with the most significant single nucleotide polymorphism (SNP) AX-94729264 on chromosome 3D was relevant to metal accumulation in wheat grains. Results of this study provide new insights into the genetic architecture of Zn accumulation in wheat grains.
微量营养素缺乏，尤其是锌（Zn）缺乏，给主要依赖谷物饮食的人带来严重的健康问题。在这里，我们使用 207 个面包小麦品种的多样性群体，进行了全基因组关联研究（GWAS），以检测小麦（ L.）籽粒中 Zn 积累的遗传基础。为了揭示控制 Zn 积累的真实数量性状位点（QTL），我们在三个地点种植了这些品种。总共鉴定出 29 个与 Zn 籽粒积累相关的独特位点。值得注意的是，在至少两个环境中检测到位于染色体 1B、3B、3D、4A、5A、5B 和 7A 上的 7 个非冗余位点。在这些数量性状位点（QTL）中，有 6 个与已知的 QTL 或基因重合，而在这项研究中鉴定出的 3D 染色体上的最高效应 QTL 以前没有报道过。对公共数据库的搜索表明，这 7 个鉴定出的 QTL 与与 Zn 积累相关的 7 个推定候选基因重合。在这 7 个基因中，与 3D 染色体上最显著的单核苷酸多态性（SNP）AX-94729264 相关的 基因（）与小麦籽粒中金属积累有关。本研究的结果为小麦籽粒 Zn 积累的遗传结构提供了新的见解。
