Park Sang Ho, Jung Bom Yee, Lee Soo Young, Yu Dong Soo, Woo So-Youn, Yun Seong-Taek, Lee Jong Tae, Kim Jin-Wou, Lee Young Bok
Clinical Research Laboratory, Uijeongbu St. Mary's Hospital, The Catholic University of Korea, Uijeongbu, Korea.
Department of Dermatology, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Ann Dermatol. 2021 Oct;33(5):440-447. doi: 10.5021/ad.2021.33.5.440. Epub 2021 Sep 8.
Background: Hot springs have been traditionally used as an alternative treatment for a wide range of diseases, including rheumatoid arthritis, bronchial asthma, diabetes, hypertension, psoriasis and atopic dermatitis. However, the clinical effects and therapeutic mechanisms associated with hot springs remain poorly defined.
The purpose of this study was to demonstrate the different effects of hot springs on cellular viability and secretion of inflammatory cytokines on keratinocyte in two geographically representative types of hot springs: NaHCO-type and NaCl-type, which are the most common types in South Korea.
We performed WST-1, BrdU measurements, human inflammatory cytokine arrays and enzyme-linked immunosorbent assay in HaCaT cells stimulated with toll-like receptor 3 by polyinosinicpolycytidylic acid.
The interaction effects of cell viability and cell proliferation were not significantly different regardless of polyinosinic-polycytidylic acid stimulation and cultured hot springs type. Cytokine array and enzymelinked immunosorbent assay analysis showed increased expression of inflammatory cytokines such as interleukin6 and granulocyte-macrophage colony-stimulating factor by polyinosinic-polycytidylic acid stimulation, with expression levels differing according to hot springs hydrochemical composition. Cytokine reduction was not significant.
The effects and mechanisms of hot springs treatment in keratinocytes were partially elucidated.
传统上，温泉一直被用作多种疾病的替代治疗方法，包括类风湿性关节炎、支气管哮喘、糖尿病、高血压、银屑病和特应性皮炎。然而，与温泉相关的临床效果和治疗机制仍不明确。
本研究旨在证明在韩国两种具有地理代表性的温泉类型（NaHCO型和NaCl型，这是韩国最常见的类型）中，温泉对角质形成细胞的细胞活力和炎性细胞因子分泌的不同影响。
我们在用聚肌苷酸-聚胞苷酸刺激Toll样受体3的HaCaT细胞中进行了WST-1、BrdU测量、人炎性细胞因子阵列分析和酶联免疫吸附测定。
无论聚肌苷酸-聚胞苷酸刺激和培养的温泉类型如何，细胞活力和细胞增殖的相互作用效应均无显著差异。细胞因子阵列分析和酶联免疫吸附测定分析表明，聚肌苷酸-聚胞苷酸刺激可使白细胞介素6和粒细胞-巨噬细胞集落刺激因子等炎性细胞因子的表达增加，且表达水平因温泉水化学组成而异。细胞因子减少不显著。
温泉治疗对角质形成细胞的作用和机制得到了部分阐明。
