Antibiotic Resistance and Virulence Genes of Isolated from Patients with Urinary Tract Infections After Kidney Transplantation from Deceased Donors.

OBJECTIVE

This study aimed to determine the prevalence of antibiotic resistance and virulence genes of strains among patients with urinary tract infections (UTIs) after kidney transplantation from deceased donors.

METHODS

Between January 2014 and June 2018, 64 patients who received kidney transplants from deceased donors at our institution developed a UTI due to . Polymerase chain reaction was used to detect virulence genes in strains. The Kirby-Bauer method was used to evaluate the antibiotic susceptibility pattern of the isolates.

RESULTS

Among the study cohort, 46 (71.9%) UTIs were community-acquired (CA), and 18 (28.1%) were hospital-acquired (HA). The percentages of isolated strains that showed antibiotic resistance were as follows: 92.2% to ampicillin, 76.6% to cefalotin, 81.3% to carbenicillin, 29.7% to ciprofloxacin, 62.5% to cotrimoxazole, 35.9% to gentamicin, 34.4% to levofloxacin, 28.1% to norfloxacin, 68.8% to pefloxacin, 57.8% to trimethoprim/sulfamethoxazole, and 20.3% to amikacin. HA showed higher resistance to ciprofloxacin, cotrimoxazole, trimethoprim/sulfamethoxazole and amikacin, compared with CA (<0.05). The most prevalent virulence genes among the strains were (64.1%), followed by (56.3%), (46.9%), (45.3%), (31.3%), (25%), (23.4%), (15.6%), (14.1%), (9.4%), and (4.7%). The and genes were found more frequently in the HA than in the CA (<0.05).

CONCLUSION

The strains, especially HA , isolated from UTI patients after kidney transplantation from deceased donors showed resistance to multiple antibiotics and harbored numerous virulence genes. These findings provide insight for genetic characterizations and epidemiological studies of strains causing UTIs in patients after kidney transplantation from deceased donors.