Cui Yaocheng, Qi Jiancheng, Cai Dongjie, Fang Jing, Xie Yue, Guo Hongrui, Chen Shiyi, Ma Xiaoping, Gou Liping, Cui Hengmin, Geng Yi, Ye Gang, Zhong Zhijun, Ren Zhihua, Hu Yanchun, Wang Ya, Deng Junliang, Yu Shuming, Cao Suizhong, Zou Huawei, Wang Zhisheng, Zuo Zhicai
Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, China.
Front Microbiol. 2021 Sep 20;12:700704. doi: 10.3389/fmicb.2021.700704. eCollection 2021.
Transportation is an inevitable phase for the cattle industry, and its effect on the respiratory system of transported cattle remains controversial. To reveal cattle's nasopharyngeal microbiota dynamics, we tracked a batch of beef calves purchased from an auction market, transported to a farm by vehicle within 3 days, and adaptively fed for 7 days. Before and after the transport and after the placement, a total of 18 nasopharyngeal mucosal samples were collected, and microbial profiles were obtained using a metagenomic shotgun sequencing approach. The diversity, composition, structure, and function of the microbiota were collected at each time point, and their difference was analyzed. The results showed that, before the transportation, there were a great abundance of potential bovine respiratory disease (BRD)-related pathogens, and the transportation did not significantly change their abundance. After the transportation, 7 days of placement significantly decreased the risk of BRD by decreasing the abundance of potential BRD-related pathogens even if the diversity was decreased. We also discussed the controversial results of transportation's effect in previous works and the decrease in diversity induced by placement. Our work provided more accurate information about the effect of transportation and the followed placement on the calf nasopharyngeal microbial community, indicated the importance of adaptive placement after long-distance transport, and is helpful to prevent BRD induced by transportation stress.
运输是养牛业不可避免的一个阶段，其对运输牛呼吸系统的影响仍存在争议。为揭示牛鼻咽微生物群的动态变化，我们追踪了一批从拍卖市场购买的肉牛犊，在3天内用车辆运至一个农场，并进行7天的适应性饲养。在运输前后以及安置后，共采集了18份鼻咽黏膜样本，并采用宏基因组鸟枪法测序方法获得微生物图谱。在每个时间点收集微生物群的多样性、组成、结构和功能，并分析其差异。结果表明，运输前存在大量潜在的牛呼吸道疾病（BRD）相关病原体，运输并未显著改变其丰度。运输后，即使多样性降低，但经过7天的安置，通过降低潜在BRD相关病原体的丰度，显著降低了BRD的风险。我们还讨论了以往研究中关于运输影响的争议性结果以及安置导致的多样性降低。我们的工作提供了关于运输及随后安置对犊牛鼻咽微生物群落影响的更准确信息，表明了长途运输后适应性安置的重要性，并有助于预防运输应激诱导的BRD。
