Department of Chemistry and The N.1 Institute for Health, National University of Singapore, Singapore, 117543, Singapore.
Paul C. Lauterbur Research Center for Biomedical Imaging, Key Laboratory for Magnetic Resonance and Multimodality Imaging of Guangdong Province, Shenzhen Key Laboratory of Ultrasound Imaging and Therapy, CAS key laboratory of health informatics, Institute of Biomedical and Health Engineering, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, P. R. China.
Adv Mater. 2021 Dec;33(49):e2102950. doi: 10.1002/adma.202102950. Epub 2021 Oct 7.
Lanthanide-based NIR-IIb nanoprobes are ideal for in vivo imaging. However, existing NIR-IIb nanoprobes often suffer from low tumor-targeting specificity, limiting their widespread use. Here the application of bioorthogonal nanoprobes with high tumor-targeting specificity for in vivo NIR-IIb luminescence imaging and magnetic resonance imaging (MRI) is reported. These dual-modality nanoprobes can enhance NIR-IIb emission by 20-fold and MRI signal by twofold, compared with non-bioorthogonal nanoprobes in murine subcutaneous tumors. Moreover, these bioorthogonal probes enable orthotopic brain tumor imaging. Implementation of bio-orthogonal chemistry significantly reduces the nanoprobe dose and hence cytotoxicity, providing a paradigm for real-time in vivo visualization of tumors.
基于镧系元素的近红外二区纳米探针非常适合用于体内成像。然而，现有的近红外二区纳米探针通常存在肿瘤靶向特异性低的问题，限制了其广泛应用。本研究报告了具有高肿瘤靶向特异性的生物正交纳米探针在活体近红外二区荧光成像和磁共振成像（MRI）中的应用。与非生物正交纳米探针相比，这些双模态纳米探针可使鼠皮下肿瘤的近红外二区发射强度增强 20 倍，磁共振信号增强 2 倍。此外，这些生物正交探针还可实现原位脑肿瘤成像。生物正交化学的实施显著降低了纳米探针的剂量和细胞毒性，为肿瘤的实时体内可视化提供了范例。
