National Medical Research Center of Cardiology, Ministry of Health of the Russian Federation, Moscow, Russia.
Bull Exp Biol Med. 2021 Sep;171(5):588-591. doi: 10.1007/s10517-021-05274-5. Epub 2021 Oct 7.
We studied the contribution of von Willebrand factor (vWF) into blood cell adhesion to collagen-coated surfaces in whole blood of healthy volunteers. Adhesion of blood cells to collagen I was measured at shear rate of 2300 sec. The interaction of platelet GPIIb/IIIa receptor with vWF was blocked with monoclonal anti-GPIIb/IIIa antibodies. The degree of cell adhesion was quantified by measuring the intensity of scattered light after 15-min perfusion: in samples with blocked GPIIb/IIIa it decreased to 0.39±0.13 V vs 0.06±0.03 V in control samples (p=0.002). Under a fluorescence microscope, intensively stained structures consisting of vWF, platelets, and leukocytes attached to the collagen surface were observed. After blockade of GPIIb/IIIa, these structures were absent. Leukocyte recruitment at high shear rates is a time-dependent process sensitive to complex interaction of vWF, leukocytes, and platelets, in which the platelet GPIIb/IIIa receptor is essential.
我们研究了在健康志愿者的全血中，血管性血友病因子（vWF）对血细胞黏附到胶原蛋白涂层表面的贡献。在剪切速率为 2300 秒的情况下测量血细胞对胶原蛋白 I 的黏附。用单克隆抗 GPIIb/IIIa 抗体阻断血小板 GPIIb/IIIa 受体与 vWF 的相互作用。通过测量 15 分钟灌注后的散射光强度来定量细胞黏附程度：在阻断 GPIIb/IIIa 的样本中，其值降至 0.39±0.13 V，而在对照样本中为 0.06±0.03 V（p=0.002）。在荧光显微镜下，可以观察到强烈染色的结构，这些结构由黏附在胶原蛋白表面的 vWF、血小板和白细胞组成。阻断 GPIIb/IIIa 后，这些结构消失。白细胞在高剪切速率下的募集是一个依赖时间的过程，对 vWF、白细胞和血小板的复杂相互作用敏感，其中血小板 GPIIb/IIIa 受体是必不可少的。
