State Key Laboratory of Tropical Oceanography, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China.
Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou, 511458, China.
Ecotoxicology. 2021 Nov;30(9):1826-1840. doi: 10.1007/s10646-021-02483-6. Epub 2021 Oct 7.
Cold events had broadly affected the survival and geographic distribution of mangrove plants. Kandelia obovata, has an excellent cold tolerance as a true halophyte and widespread mangrove species. In this study, physiological characters and comparative proteomics of leaves of K. obovata were performed under cold treatment. The physiological analysis showed that K. obovata could alleviate its cold-stress injuries through increasing the levels of antioxidants, the activities of related enzymes, as well as osmotic regulation substances (proline). It was detected 184 differentially expressed protein spots, and of 129 (70.11%) spots were identified. These proteins have been involved in several pathways such as the stress and defense, photosynthesis and photorespiration, signal transduction, transcription factors, protein biosynthesis and degradation, molecular chaperones, ATP synthesis, the tricarboxylic acid (TCA) cycle and primary metabolisms. The protein post-translational modification may be a common phenomenon and plays a key role in cold-response process in K. obovata. According to our precious work, a schematic diagram was drawn for the resistance or adaptation strategy of mangrove plants under cold stress. This study provided valuable information to understand the mechanism of cold tolerance of K. obovata.
冷事件广泛影响了红树林植物的生存和地理分布。秋茄（Kandelia obovata）作为一种真正的盐生植物和广泛分布的红树林物种，具有出色的耐寒性。在这项研究中，对秋茄叶片进行了冷处理下的生理特征和比较蛋白质组学分析。生理分析表明，秋茄可通过增加抗氧化剂水平、相关酶的活性以及渗透调节物质（脯氨酸）来减轻冷胁迫损伤。共检测到 184 个差异表达的蛋白点，其中 129 个（70.11%）蛋白点得到鉴定。这些蛋白参与了多种途径，如应激和防御、光合作用和光呼吸、信号转导、转录因子、蛋白质生物合成和降解、分子伴侣、ATP 合成、三羧酸（TCA）循环和初级代谢等。蛋白质翻译后修饰可能是一种普遍现象，在秋茄的冷响应过程中起着关键作用。根据我们的研究工作，绘制了一幅红树林植物在冷胁迫下的抗性或适应策略示意图。本研究为了解秋茄的耐寒机制提供了有价值的信息。
