Department of Plastic and Reconstructive Surgery, Peking University 3rd Hospital, NO.49 of North Huayuan Road, Beijing, China.
Tissue Eng Regen Med. 2022 Feb;19(1):1-9. doi: 10.1007/s13770-021-00394-5. Epub 2021 Oct 7.
As a contour-supporting material, the cartilage has a significant application value in plastic surgery. Since the development of hydrogel scaffolds with sufficient biomechanical strength and high biocompatibility, cell-laden hydrogels have been widely studied for application in cartilage bioengineering. This systematic review summarizes the latest research on engineered cartilage constructed using cell-laden hydrogel scaffolds in plastic surgery.
A systematic review was performed by searching the PubMed and Web of Science databases using selected keywords and Medical Subject Headings search terms.
Forty-two studies were identified based on the search criteria. After full-text screening for inclusion and exclusion criteria, 18 studies were included. Data collected from each study included culturing form, seed cell types and sources, concentration of cells and gels, scaffold materials and bio-printing structures, and biomechanical properties of cartilage constructs. These cell-laden hydrogel scaffolds were reported to show some feasibility of cartilage engineering, including better cell proliferation, enhanced deposition of glycosaminoglycans and collagen type II in the extracellular matrix, and better biomechanical properties close to the natural state.
Cell-laden hydrogels have been widely used in cartilage bioengineering research. Through 3-dimensional (3D) printing, the cell-laden hydrogel can form a bionic contour structure. Extracellular matrix expression was observed in vivo and in vitro, and the elastic modulus was reported to be similar to that of natural cartilage. The future direction of cartilage tissue engineering in plastic surgery involves the use of novel hydrogel materials and more advanced 3D printing technology combined with biochemistry and biomechanical stimulation.
作为一种轮廓支撑材料，软骨在整形外科学中有重要的应用价值。自从具有足够的生物力学强度和高生物相容性的水凝胶支架发展以来，细胞负载的水凝胶已被广泛研究应用于软骨生物工程。本系统综述总结了在整形外科学中使用细胞负载水凝胶支架构建工程软骨的最新研究。
通过使用选定的关键词和医学主题词搜索术语在 PubMed 和 Web of Science 数据库中进行系统综述。
根据搜索标准确定了 42 项研究。经过全文筛选纳入和排除标准，有 18 项研究被纳入。从每项研究中收集的数据包括培养形式、种子细胞类型和来源、细胞和凝胶的浓度、支架材料和生物打印结构以及软骨构建体的生物力学特性。这些细胞负载水凝胶支架被报道显示出一些软骨工程的可行性，包括更好的细胞增殖、增强细胞外基质中糖胺聚糖和 II 型胶原的沉积，以及更接近自然状态的更好的生物力学特性。
细胞负载水凝胶已广泛应用于软骨生物工程研究。通过 3 维（3D）打印，细胞负载水凝胶可以形成仿生轮廓结构。在体内和体外观察到细胞外基质的表达，报道的弹性模量与天然软骨相似。整形外科学中软骨组织工程的未来方向涉及使用新型水凝胶材料和更先进的 3D 打印技术，结合生物化学和生物力学刺激。
