Aragon de Kirbach E D, Willers I, Singh S, Goedde H
Clin Genet. 1986 May;29(5):369-73. doi: 10.1111/j.1399-0004.1986.tb00506.x.
Skin fibroblasts of patients with Duchenne muscular dystrophy were analysed for HLA tissue antigens. For the examination of the antigens two modified methods were used: the microdroplet cytotoxicity assay on fibroblast suspension and the cytotoxicity assay on fibroblast monolayer cultures using fluorochromasia. The HLA determination on the fibroblast suspensions of the patients showed a variety of unspecific HLA expressions which were not found in fibroblasts of healthy controls. These reactions were not apparent in the test in monolayer cultures. The exact cause has not been elucidated yet, but it appears to have a strong relation to some membrane abnormality in cells of DMD patients. The reproducible lack of unspecific reactions on monolayer cultures lead us to propose that these expressions arise from artificial injuries to membranes of these patient cells during trypsination or centrifugation. The validity of this test has been confirmed by a blind study.
对杜兴氏肌肉营养不良症患者的皮肤成纤维细胞进行了 HLA 组织抗原分析。为检测这些抗原，使用了两种改良方法：对成纤维细胞悬液的微量滴度细胞毒性试验，以及使用荧光染色法对成纤维细胞单层培养物进行的细胞毒性试验。对患者成纤维细胞悬液的 HLA 测定显示出多种非特异性 HLA 表达，而在健康对照者的成纤维细胞中未发现这些表达。这些反应在单层培养试验中并不明显。确切原因尚未阐明，但似乎与 DMD 患者细胞中的某些膜异常有很强的关系。单层培养物中可重复出现的非特异性反应缺失使我们提出，这些表达是在胰蛋白酶消化或离心过程中这些患者细胞的膜受到人为损伤所致。这项检测的有效性已通过一项盲法研究得到证实。
