Schultz R M, Chirigos M A
Cancer Res. 1978 Apr;38(4):1003-7.
Lymphokine preparations, including supernatants derived from antigen-stimulated Bacillus Calmette-Guérin-immune spleen cell cultures and normal spleen cells incubated with insoluble concanavalin A, were compared with partially purified L-cell interferon for the ability to render resting macrophages nonspecifically tumoricidal in vitro. Significant activation of macrophages by lymphokine preparations occurred at concentrations as low as 0.5 and 0.25% of the assay mixture for antigen-stimulated and concanavalin A-induced lymphokine, respectively. These end point concentrations were each determined to contain 0.3 unit of interferon per ml. Supernatants obtained from unstimulated normal spleen cells, concanavalin A-treated nu/nu spleen cells, or Bacillus Calmette-Guérin-immune spleen cells in the absence of sensitizing antigen did not enhance macrophage tumoricidal function and lacked interferon. Activation by L-cell interferon required at least 1 unit/ml. The macrophage-activating factors contained in lymphokine and interferon preparations were stable at pH 2 and at 56 degrees, but they were destroyed when heated at 80 degrees for 30 min, and were inactivated by trypsin. The data demonstrate common properties for the induction of tumoricidal macrophages by these divese preparations.
将来自抗原刺激的卡介苗免疫脾细胞培养物的上清液以及与不溶性伴刀豆球蛋白A孵育的正常脾细胞等淋巴因子制剂，与部分纯化的L细胞干扰素进行比较，以评估它们在体外使静息巨噬细胞产生非特异性杀肿瘤作用的能力。对于抗原刺激的淋巴因子和伴刀豆球蛋白A诱导的淋巴因子，分别在测定混合物浓度低至0.5%和0.25%时，巨噬细胞被淋巴因子制剂显著激活。这些终点浓度经测定每毫升均含有0.3单位干扰素。从未刺激的正常脾细胞、伴刀豆球蛋白A处理的无胸腺裸鼠脾细胞或无致敏抗原时的卡介苗免疫脾细胞获得的上清液，均未增强巨噬细胞的杀肿瘤功能且缺乏干扰素。L细胞干扰素激活巨噬细胞至少需要1单位/毫升。淋巴因子和干扰素制剂中含有的巨噬细胞激活因子在pH2和56℃时稳定，但在80℃加热30分钟会被破坏，且会被胰蛋白酶灭活。数据表明这些不同制剂在诱导杀肿瘤巨噬细胞方面具有共同特性。
