Pace J L, Russell S W, Schreiber R D, Altman A, Katz D H
Proc Natl Acad Sci U S A. 1983 Jun;80(12):3782-6. doi: 10.1073/pnas.80.12.3782.
Antiviral and macrophage-priming activities in the supernatant medium of a subclone of a concanavalin A-stimulated mouse T-cell hybridoma were investigated. The two activities were associated with a molecular weight of approximately 50,000 and could not be separated by various approaches. Both activities were eliminated by a highly specific neutralizing antibody against mouse interferon-gamma, but not by antibody against interferon-alpha and -beta. The ratio of priming to antiviral activity in the hybridoma culture supernate was indistinguishable from the ratio obtained with mouse interferon-gamma prepared by recombinant DNA technology. It was concluded from these data that the priming activity in hybridoma culture supernates was attributable to interferon-gamma and that this mediator is one form of the lymphokine macrophage-activating factor. Interferon-gamma was greater than 800 times more efficient at priming mouse macrophages for tumor cell killing than was a mixture of interferon-alpha and -beta. This finding contributes to growing awareness that type II interferon may have greater immunoregulatory potential than type I interferons.
对伴刀豆球蛋白A刺激的小鼠T细胞杂交瘤亚克隆的上清培养基中的抗病毒和巨噬细胞启动活性进行了研究。这两种活性与分子量约为50,000相关，并且不能通过各种方法分离。两种活性均被针对小鼠干扰素-γ的高度特异性中和抗体消除，但未被针对干扰素-α和-β的抗体消除。杂交瘤培养上清液中启动活性与抗病毒活性的比率与通过重组DNA技术制备的小鼠干扰素-γ所获得的比率无法区分。从这些数据得出的结论是，杂交瘤培养上清液中的启动活性归因于干扰素-γ，并且这种介质是淋巴因子巨噬细胞激活因子的一种形式。与干扰素-α和-β的混合物相比，干扰素-γ在启动小鼠巨噬细胞杀伤肿瘤细胞方面的效率要高800倍以上。这一发现有助于人们越来越意识到II型干扰素可能比I型干扰素具有更大的免疫调节潜力。
