State Key Laboratory of Hybrid Rice, Hongshan Laboratory of Hubei Province, Key Laboratory for Research and Utilization of Heterosis in Indica Rice of Ministry of Agriculture, Engineering Research Center for Plant Biotechnology and Germplasm Utilization of Ministry of Education, College of Life Science, Wuhan University, Wuhan, 430072, China.
State Key Laboratory of Hybrid Rice, Hongshan Laboratory of Hubei Province, Key Laboratory for Research and Utilization of Heterosis in Indica Rice of Ministry of Agriculture, Engineering Research Center for Plant Biotechnology and Germplasm Utilization of Ministry of Education, College of Life Science, Wuhan University, Wuhan, 430072, China.
Plant Sci. 2021 Nov;312:111057. doi: 10.1016/j.plantsci.2021.111057. Epub 2021 Sep 16.
Plant fertility and resistance to stress environments are antagonistic to each other. At booting stage, fertility is often sacrificed for survive in rice under abiotic stress. However, the relationship between fertility and resistance at molecular level remains elusive. Here, we identified a transcription factor, OsAlfin like 5, which regulates the OsTMS5 and links both the drought stress response and thermosensitive genic male sterility. The OsAL5 overexpression plants (OE-OsAL5) became sensitive to temperature owning to the OsTMS5 that the OE-OsAL5 plants were fertile under low temperature (23 °C) and sterile under high temperature (28 °C). Significantly, the survival rate of OE-OsAL5 lines was higher than that of the wide-type (WT) under drought stress. Further experiments confirmed that the OsAL5 regulated both of the OsTMS5 and the down-stream drought-related genes by binding to the 'GTGGAG' element in vivo, revealing that the OsAL5 participated both in the drought stress response and thermosensitive genic male sterility in rice. These findings open up the possibility of breeding elite TGMS lines with strong drought tolerance by manipulating the expression of OsAL5.
植物的育性和对胁迫环境的抗性是相互拮抗的。在抽穗期，水稻在非生物胁迫下常常为了生存而牺牲育性。然而，育性和抗性在分子水平上的关系仍然难以捉摸。在这里，我们鉴定了一个转录因子 OsAlfin like 5，它调节 OsTMS5，并连接干旱胁迫响应和热敏雄性不育。由于 OsTMS5 的存在，过表达 OsAL5 的植物（OE-OsAL5）对温度敏感，OE-OsAL5 植物在低温（23°C）下具有育性，而在高温（28°C）下不育。显著的是，OE-OsAL5 系在干旱胁迫下的存活率高于野生型（WT）。进一步的实验证实，OsAL5 通过与体内的“GTGGAG”元件结合来调节 OsTMS5 和下游与干旱相关的基因，表明 OsAL5 参与了水稻的干旱胁迫响应和热敏雄性不育。这些发现为通过操纵 OsAL5 的表达来培育具有强耐旱性的优良 TGMS 系开辟了可能性。
