Hotchkiss Brain Institute and the Department of Clinical Neuroscience, University of Calgary, Calgary, AB T2N 4N1, Canada.
STAR Protoc. 2021 Sep 27;2(4):100853. doi: 10.1016/j.xpro.2021.100853. eCollection 2021 Dec 17.
Oxidized phosphatidylcholine (OxPC) found in multiple sclerosis brain lesions mediates neurodegeneration. Microglia are prominent responders to the OxPC insult, and thus, studying their protective or noxious functions is important to help halt neurodegeneration. Here, we present protocols including cell isolation and culture, animal surgeries, as well as tissue processing and isolation to study the microglia response to OxPC-mediated neurodegeneration and . For complete details on the use and execution of this protocol, please refer to Dong et al. (2021).
氧化磷脂酰胆碱（OxPC）存在于多发性硬化症的脑损伤中，介导神经退行性变。小胶质细胞对 OxPC 损伤有明显的反应，因此，研究它们的保护或有害功能对于阻止神经退行性变非常重要。在这里，我们提供了包括细胞分离和培养、动物手术以及组织处理和分离在内的方案，以研究小胶质细胞对 OxPC 介导的神经退行性变的反应。有关该方案使用和实施的完整详细信息，请参考 Dong 等人（2021 年）。
