基于流式细胞术的分析小鼠小胶质细胞呼吸链功能的方案。
Flow-cytometry-based protocol to analyze respiratory chain function in mouse microglia.
机构信息
Institute of Neuropathology, University of Freiburg, Freiburg, Germany.
Berta-Ottenstein-Programme, Faculty of Medicine, University of Freiburg, Freiburg im Breisgau, Germany.
出版信息
STAR Protoc. 2022 Feb 18;3(1):101186. doi: 10.1016/j.xpro.2022.101186. eCollection 2022 Mar 18.
Most of the protocols to analyze metabolic features of cell populations from different tissues rely on cell culture conditions. Here, we present a flow-cytometry-based protocol for measuring the respiratory chain function in permeabilized mouse microglia . We describe microglial cell isolation, followed by analyzing complex I and II using flow cytometry. This optimized protocol requires a low input of permeabilized cells and can be applied to other isolated cells or cells derived from cell cultures. For complete details on the use and execution of this protocol, please refer to Erny et al. (2021).
大多数用于分析来自不同组织的细胞群体代谢特征的方案都依赖于细胞培养条件。在这里，我们提出了一种基于流式细胞术的测定小鼠小胶质细胞呼吸链功能的方案。我们描述了小胶质细胞的分离，然后使用流式细胞术分析复合物 I 和 II。该优化方案需要低输入的通透细胞，可应用于其他分离细胞或细胞培养物衍生的细胞。有关此方案使用和执行的完整详细信息，请参阅 Erny 等人。（2021 年）。
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