U.S. Geological Survey, Eastern Ecological Science Center at the Leetown Research Laboratory, 11649 Leetown Road, Kearneysville, West Virginia, 25430, USA.
U.S. Geological Survey, West Virginia Cooperative Fish and Wildlife Research Unit, West Virginia University, Davis College of Agriculture, Natural Resources and Design, 313C Percival Hall, Morgantown, West Virginia, 26506, USA.
J Aquat Anim Health. 2022 Mar;34(1):12-19. doi: 10.1002/aah.10144. Epub 2021 Oct 8.
Histopathological assessments of young-of-the-year (age-0) Smallmouth Bass Micropterus dolomieu in the Susquehanna River drainage identified a high prevalence of the myxozoan Myxobolus inornatus. This myxozoan infects the connective tissue of the muscle below the skin but is sometimes observed in the esophagus and buccal cavity. In some instances, shallow infections cause breaks in the skin, which could increase the chance of opportunistic bacterial infections. Several microbial pathogens, including Flavobacterium columnare, Aeromonas spp., and Largemouth Bass virus, have also been cultured from clinically diseased young of year. A multiplex fluorescence in situ hybridization (FISH) assay was developed to determine potential colocalization of M. inornatus, Flavobacterium spp., and Aeromonas spp. infections. With FISH, 75% of age-0 Smallmouth Bass exhibited M. inornatus infections, 10% had Aeromonas spp. infections, and 5% had Flavobacterium spp. infections, while 3% had coinfections with both bacterial species and M. inornatus. The results of the multiplex FISH assay revealed a low occurrence of coinfections of Flavobacterium spp. and/or Aeromonas spp. with M. inornatus in randomly sampled individuals.
对萨斯奎哈纳河水系的幼年小口黑鲈（年龄 0）进行组织病理学评估，发现粘孢子虫 Myxobolus inornatus 的感染率很高。这种粘孢子虫感染皮肤下的结缔组织，但有时也会在食道和口腔中观察到。在某些情况下，浅层感染会导致皮肤破裂，这可能会增加机会性细菌感染的机会。从临床患病的幼年鱼中还培养出了几种微生物病原体，包括柱状黄杆菌、气单胞菌属和大口黑鲈病毒。已经开发出一种多重荧光原位杂交（FISH）检测方法来确定 M. inornatus、Flavobacterium spp. 和 Aeromonas spp. 感染的潜在共定位。通过 FISH 检测，75%的年龄 0 小口黑鲈存在 M. inornatus 感染，10%存在气单胞菌属感染，5%存在黄杆菌属感染，而 3%存在这两种细菌与 M. inornatus 的混合感染。多重 FISH 检测的结果显示，在随机抽样的个体中，Flavobacterium spp. 和/或 Aeromonas spp. 与 M. inornatus 的混合感染发生率较低。
