Ksepka Steven P, Rash Jacob M, Bullard Stephen A
Aquatic Parasitology Laboratory, School of Fisheries, Aquaculture, and Aquatic Sciences, College of Agriculture, Auburn University, 203 Swingle Hall, Auburn, AL 36849, USA.
North Carolina Wildlife Resources Commission, 645 Fish Hatchery Road, Marion, NC 28752, USA.
Parasitol Int. 2022 Dec;91:102615. doi: 10.1016/j.parint.2022.102615. Epub 2022 Jul 7.
Two new species of Myxobolus Bütschli, 1882 (Bivalvulida: Myxobolidae) are described from the gill and scales of smallmouth bass (Micropterus dolomieu Lacepède, 1802 [Centrarchiformes: Centrarchidae]) from the Watauga River, French Broad River Basin, North Carolina, United States. Myxobolus intralamina n. sp. infects the lumen of the lamellar arterioles and Myxobolus infrabractea n. sp. infects the inner surface of the scale. They differ from all congeners by a combination of myxospore dimensions, polar tubule coil count, and the presence or absence of an iodinophilic vacuole in the sporoplasm and an intercapsular process. A phylogenetic analysis of the small subunit ribosomal DNA (SSU rDNA) recovered M. intralamina n. sp. sister to Myxobolus lepomis and Myxobolus branchiarum and M. infrabractea n. sp. sister to Myxobolus micropterii in a clade composed of five Myxobolus spp. infecting centrarchids and Henneguya spp. (Myxobolidae) infecting percids. Histological sections of infected gill revealed intra-lamellar plasmodia of M. intralamina n. sp. within the lumen of the lamellar arterioles and plasmodia of M. infrabractea n. sp. developing beneath the scales. These new species comprise the first species of Myxobolus reported from a black bass (Micropterus Lacepède, 1802) in the Southeast United States.
从美国北卡罗来纳州法国布罗德河流域瓦托加河的小口黑鲈（Micropterus dolomieu Lacepède，1802 [鲈形目：太阳鱼科]）的鳃和鳞片中发现了两种新的粘体虫属（Myxobolus Bütschli，1882）（双壳目：粘体科）物种。层内粘体虫（Myxobolus intralamina）新种感染鳃小片动脉的管腔，鳞片下粘体虫（Myxobolus infrabractea）新种感染鳞片内表面。它们在粘孢子尺寸、极丝盘绕数、孢子质中嗜碘泡的有无以及囊间突等方面的组合与所有同属物种不同。对小亚基核糖体DNA（SSU rDNA）的系统发育分析显示，层内粘体虫新种是粘体虫属的lepomis种和鳃粘体虫（Myxobolus branchiarum）的姐妹种，鳞片下粘体虫新种是粘体虫属的micropterii种的姐妹种，它们位于一个由感染太阳鱼科鱼类的五个粘体虫属物种和感染鲈科鱼类的亨内古亚属（Henneguya）物种（粘体科）组成的进化枝中。受感染鳃的组织学切片显示，层内粘体虫新种的层内疟原虫存在于鳃小片动脉的管腔内，鳞片下粘体虫新种的疟原虫在鳞片下发育。这些新物种是美国东南部黑鲈（Micropterus Lacepède，1802）中首次报道的粘体虫属物种。
