State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China.
University of Chinese Academy of Sciences, Beijing 100049, China; School of Geographical Sciences, Fujian Normal University, Fuzhou 350007, China.
Sci Total Environ. 2022 Feb 10;807(Pt 1):150781. doi: 10.1016/j.scitotenv.2021.150781. Epub 2021 Oct 5.
Scarab larvae (Protaetia brevitarsis) could transform large quantities of agricultural waste into compost, providing a promising bio-fertilizer for soil management. There is an urgent need to assess the risk of antibiotic resistance genes (ARGs) in soil-vegetable system with application of compost derived from P. brevitarsis larvae. We conducted a pot experiment to compare the changes of ARGs in the soil and lettuce by adding four types of manure, livestock manure (chicken and swine manure) and the corresponding larval frass. Significantly low numbers of ARGs and mobile genetic elements (MGEs) were detected in both larval frass compared with the corresponding livestock manure. Pot experiment showed that the detected numbers of ARGs and MGEs in bulk soil, rhizosphere soil, and root endophytes were significantly lower in the frass-amended treatments than the raw manure-amended treatments. Furthermore, the relative abundance of ARGs and MGEs with application of chicken-frass was significant lower in rhizosphere soil and leaf endophyte. Using non-metric multidimensional scaling analysis, the patterns of soil ARGs and MGEs with chicken-frass application were more close to those from the bulk soil in the control. Structural equation models indicated that livestock manure addition was the main driver shaping soil ARGs with raw manure application, while MGEs were the key drivers in frass-amended treatments. These findings demonstrated that application of livestock manure vermicomposting via scarab larvae (P. brevitarsis) may be at low risk in spreading manure-borne ARGs through soil-plant system, providing an alternative technique for reducing ARGs in organic waste.
蜣螂幼虫（Protaetia brevitarsis）可以将大量农业废弃物转化为堆肥，为土壤管理提供有前景的生物肥料。迫切需要评估应用蜣螂幼虫粪便制成的堆肥对土壤-蔬菜系统中抗生素抗性基因（ARGs）的风险。我们进行了一项盆栽实验，比较了添加四种粪肥（鸡粪和猪粪）和相应的幼虫粪肥后土壤和生菜中 ARGs 的变化。与相应的牲畜粪便相比，幼虫粪肥中 ARGs 和可移动遗传元件（MGEs）的数量明显较低。盆栽实验表明，在添加幼虫粪肥的处理中，土壤、根际土壤和根内生菌中 ARGs 和 MGEs 的检出数量明显低于原始粪肥处理。此外，应用鸡粪肥后，根际土壤和叶内生菌中 ARGs 和 MGEs 的相对丰度显著降低。利用非度量多维尺度分析，应用鸡粪肥后土壤 ARGs 和 MGEs 的模式与对照中土壤的模式更为接近。结构方程模型表明，原始粪肥的添加是通过土壤中原始粪肥应用塑造土壤 ARGs 的主要驱动因素，而 MGEs 是幼虫粪肥处理的关键驱动因素。这些发现表明，通过蜣螂幼虫（P. brevitarsis）进行的牲畜粪便生物转化，在通过土壤-植物系统传播粪肥来源的 ARGs 方面风险较低，为减少有机废物中的 ARGs 提供了一种替代技术。
