School of Public Health and Management, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China.
School of Public Health and Management, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China; Key Laboratory of Watershed Science and Health Risk of Zhejiang Province, Wenzhou Medical University, Wenzhou 325035, China.
Transpl Immunol. 2021 Dec;69:101481. doi: 10.1016/j.trim.2021.101481. Epub 2021 Oct 5.
Increasing evidence has demonstrated the functional relevance of long non-coding RNAs (lncRNAs) to the occurrence and progression of tumors. However, the role of lncRNA FAM83C-AS1 in tumors remains unclear. We aim to prove the relationship between FAM83C-AS1 and colon cancer (CCa).
The expression level of FAM83C-AS1 in CCa were explored through bioinformatics analysis. Receiver operating characteristic (ROC) curves, Kaplan-Meier method and Cox regression analysis were used to evaluated to its roles in the prognosis of CCa. The biological function was explored through gene set enrichment analysis (GSEA). The correlation between FAM83C-AS1 expression and immune infiltration and immunotherapy response was analyzed through single-sample GSEA (ssGSEA) and Spearman correlation.
FAM83C-AS1 was up-regulated in multiple cancers including CCa. Increased FAM83C-AS1 expression in CCa was correlated with advanced clinical pathologic characteristics (histological type, M stage; p < 0.05). ROC curve suggested the significant diagnostic and prognostic ability of FAM83C-AS1 (AUC = 0.759). High FAM83C-AS1 expression predicted a poorer overall survival (OS, p = 0.033) and disease-free survival (DFS, p = 0.043), and FAM83C-AS1 expression was independently correlated with OS in CCa patients (HR:1.69; 95% CI:1.07-2.67; p = 0.024). In addition, GSEA suggested the involvement of FAM83C-AS1 in immune-related pathways. We also found the correlation of FAM83C-AS1 with DC cells and T helper cells and a potential of the measurement in response to immunotherapy.
High expression of FAM83C-AS1 indicated poor prognosis and correlation with immune infiltrations and immune response in CCa, and it may be a promising biomarker of prognosis and response to immunotherapy for CCa.
越来越多的证据表明长链非编码 RNA（lncRNA）在肿瘤的发生和发展中具有功能相关性。然而，lncRNA FAM83C-AS1 在肿瘤中的作用尚不清楚。本研究旨在证实 FAM83C-AS1 与结肠癌（CCa）之间的关系。
通过生物信息学分析探讨 FAM83C-AS1 在 CCa 中的表达水平。采用受试者工作特征（ROC）曲线、Kaplan-Meier 方法和 Cox 回归分析评估 FAM83C-AS1 对 CCa 预后的作用。通过基因集富集分析（GSEA）探讨其生物学功能。通过单样本 GSEA（ssGSEA）和 Spearman 相关性分析 FAM83C-AS1 表达与免疫浸润和免疫治疗反应的相关性。
FAM83C-AS1 在多种癌症中上调，包括 CCa。在 CCa 中，FAM83C-AS1 表达增加与更晚期的临床病理特征（组织学类型、M 分期；p<0.05）相关。ROC 曲线表明 FAM83C-AS1 具有显著的诊断和预后能力（AUC=0.759）。FAM83C-AS1 高表达预示着更差的总生存（OS，p=0.033）和无病生存（DFS，p=0.043），并且在 CCa 患者中，FAM83C-AS1 表达与 OS 独立相关（HR：1.69；95%CI：1.07-2.67；p=0.024）。此外，GSEA 表明 FAM83C-AS1 参与了免疫相关途径。我们还发现 FAM83C-AS1 与树突状细胞和辅助性 T 细胞相关，并且可能成为评估免疫治疗反应的一个有潜力的标志物。
FAM83C-AS1 高表达提示 CCa 预后不良，与免疫浸润和免疫反应相关，可能是 CCa 预后和免疫治疗反应的有前途的标志物。
