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乳酸菌、糖蜜及其组合对木薯叶青贮发酵品质和细菌群落的影响。

Effect of lactic acid bacteria, molasses, and their combination on the fermentation quality and bacterial community of cassava foliage silage.

机构信息

Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan, China.

Key Laboratory of Ministry of Education for Genetics and Germplasm Innovation of Tropical Special Trees and Ornamental Plants, Key Laboratory of Germplasm Resources of Tropical Special Ornamental Plants of Hainan Province, College of Forestry, College of Tropical Crops, Hainan University, Danzhou, Hainan, China.

出版信息

Anim Sci J. 2021 Jan-Dec;92(1):e13635. doi: 10.1111/asj.13635.

Abstract

This study investigated the effects of LAB inoculants (L) and molasses (M) on the microbial community and fermentation quality of cassava foliage (CF). The small segments (about 2-3 cm) CF were ensiled in plastic bags and incubated at normal temperature (25°C). Four treatments were carried out as follows: control (no additives, CK), LAB inoculants (Lactobacillus plantarum, L), molasses (M), and LAB in combination with molasses (LM). The LAB and molasses obviously altered the bacterial community structure of the CF silage and enhanced the fermentation quality. The combination addition could increase the abundance of Lactobacillus and reduce the Pseudomonas. The LAB and molasses also significantly elevated the lactic acid concentration (P < 0.001) and decreased the pH (P < 0.001), as well as the concentrations of acetic acid, propionic acid, butyric acid, and ammonia-N (P < 0.05). In addition, the combination treatment displayed more effective results on silage fermentation. The LAB and molasses improved the fermentation quality of the CF silage by altering the bacterial community structure. Furthermore, the bacterial community was significantly correlated with fermentation indexes.

摘要

本研究探讨了 LAB 接种剂(L)和糖蜜(M)对木薯叶(CF)微生物群落和发酵品质的影响。将小段(约 2-3cm)CF 青贮在塑料袋中,在常温(25°C)下进行培养。进行了以下四项处理:对照(无添加剂,CK)、LAB 接种剂(植物乳杆菌,L)、糖蜜(M)和 LAB 与糖蜜的组合(LM)。LAB 和糖蜜明显改变了 CF 青贮的细菌群落结构,并提高了发酵品质。组合添加可以增加乳酸菌的丰度,减少假单胞菌的数量。LAB 和糖蜜还显著提高了乳酸浓度(P<0.001),降低了 pH 值(P<0.001),以及乙酸、丙酸、丁酸和氨态氮的浓度(P<0.05)。此外,组合处理对青贮发酵的效果更为显著。LAB 和糖蜜通过改变细菌群落结构提高了 CF 青贮的发酵品质。此外,细菌群落与发酵指标显著相关。

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