Departments of Medicinal Chemistry, Biology and Bioengineering, University of Utah, Salt Lake City, UT, USA.
Department of Neurobiology, & Department of Nutrition & Integrative Physiology, University of Utah, Salt Lake City, UT, USA.
Methods Mol Biol. 2022;2303:595-603. doi: 10.1007/978-1-0716-1398-6_45.
Xylosides are small synthetic molecules consisting of a xylose molecule attached to an aglycone group and serve as primers in the assembly of core protein free glycosaminoglycans using cellular machinery. Synthetic xylosides hold great promise in many biomedical applications and as therapeutics. Recent advances in the study of xylosides have opened up the possibility of developing xylosides as therapeutics to achieve a desirable biological outcome through their selective priming and inhibitory activities toward glycosaminoglycan biosynthesis. The approach described, herein, will serve as a general strategy to comprehensively screen xylosides and evaluate their ability to promote or inhibit angiogenesis, a critical biological process that is dysregulated in over 70 human diseases.
木糖苷是由一个木糖分子连接到一个非糖基团上的小分子合成分子，它们作为细胞机制中核心蛋白游离糖胺聚糖组装的引物。合成木糖苷在许多生物医学应用和治疗中具有巨大的应用前景。最近对木糖苷的研究进展为开发木糖苷作为治疗剂提供了可能性，通过它们对糖胺聚糖生物合成的选择性启动和抑制活性来实现理想的生物学结果。本文所述的方法将作为一种通用策略，全面筛选木糖苷，并评估它们促进或抑制血管生成的能力，血管生成是超过 70 种人类疾病中失调的关键生物学过程。
