木糖苷在干细胞龛调控中的应用以调节人神经干细胞分化和神经突生长。

Applications of Xylosides in the Manipulation of Stem Cell Niche to Regulate Human Neural Stem Cell Differentiation and Neurite Outgrowth.

机构信息

Departments of Biology, Bioengineering and Medicinal Chemistry, University of Utah, Salt Lake City, UT, USA.

Department of Neurobiology & Department of Nutrition and Integrative Physiology, University of Utah, Salt Lake City, UT, USA.

出版信息

Methods Mol Biol. 2022;2303:779-788. doi: 10.1007/978-1-0716-1398-6_58.

DOI:10.1007/978-1-0716-1398-6_58

PMID:34626422

Abstract

The extracellular matrix (ECM) plays a pivotal role in the regulation of neural stem cell differentiation, axon guidance and growth, and neural plasticity. Glycosaminoglycans, such as heparan sulfate and chondroitin sulfate, are significant components of brain ECM that dictates neurogenesis and neural repair. Herein, we describe a simple method to assess the effect of xylsoides, which serve as primers and inhibitors of GAG biosynthesis, on human neural stem cell differentiation and neurite outgrowth in in vitro culture conditions.

摘要

细胞外基质（ECM）在调节神经干细胞分化、轴突导向和生长以及神经可塑性方面起着关键作用。糖胺聚糖（如肝素硫酸盐和软骨素硫酸盐）是脑 ECM 的重要组成部分，决定着神经发生和神经修复。在此，我们描述了一种简单的方法来评估木二糖类似物（作为 GAG 生物合成的引物和抑制剂）对人神经干细胞在体外培养条件下的分化和突起生长的影响。