Babiss L E, Bennett A, Friedman J M, Darnell J E
Proc Natl Acad Sci U S A. 1986 Sep;83(17):6504-8. doi: 10.1073/pnas.83.17.6504.
As tested by DNase I digestion, the chromatin structure in several regions 5' to the rat serum albumin gene varies in tissues and cell lines that differ in transcription rate of this gene. Three DNase I-hypersensitive regions were found in hepatocyte nuclei but not in kidney cell nuclei. The sites were approximately 2.8 kbp (site 1), 0.2 kbp (site 2), and 0.05 kbp (site 3) upstream from the cap site of the gene. In rat fetal liver tissue and rat hepatoma cell lines (FaO, C2, and C2-rev7), as well as in cultured primary hepatocytes where the rate of albumin gene transcription is lower than in adult liver, hypersensitive site (HSS) 1 was absent while sites 2 and 3 were present. In addition, the C2 cell line, which does not express albumin mRNA, contains a different HSS at position -1.5 kbp. Factors (proteins) bound to sites 2 and 3 may allow cell-specific transcription, but the additional factor interaction at site 1 could be required for a maximal rate of albumin gene transcription.
经DNA酶I消化试验检测，大鼠血清白蛋白基因5'端几个区域的染色质结构在该基因转录速率不同的组织和细胞系中有所差异。在肝细胞核中发现了三个对DNA酶I敏感的区域，而在肾细胞核中未发现。这些位点位于该基因帽位点上游约2.8千碱基对（位点1）、0.2千碱基对（位点2）和0.05千碱基对（位点3）处。在大鼠胎儿肝脏组织和大鼠肝癌细胞系（FaO、C2和C2-rev7）中，以及在白蛋白基因转录速率低于成年肝脏的原代培养肝细胞中，敏感位点（HSS）1不存在，而位点2和3存在。此外，不表达白蛋白mRNA的C2细胞系在-1.5千碱基对位置含有一个不同的HSS。与位点2和3结合的因子（蛋白质）可能允许细胞特异性转录，但位点1处额外的因子相互作用可能是白蛋白基因最大转录速率所必需的。
