使用线粒体蛋白序列数据推断来自有袋目和袋熊目的袋形目线虫亚科线虫的系统发育关系。

Phylogenetic relationships of the nematode subfamily Phascolostrongylinae from macropodid and vombatid marsupials inferred using mitochondrial protein sequence data.

机构信息

Department of Veterinary Biosciences, Melbourne Veterinary School, The University of Melbourne, Victoria, Australia.

South Australian Museum, Adelaide, South Australia, Australia.

出版信息

Parasit Vectors. 2021 Oct 9;14(1):523. doi: 10.1186/s13071-021-05028-2.

DOI:10.1186/s13071-021-05028-2

PMID:34627345

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8501563/

Abstract

BACKGROUND

The subfamily Phascolostrongylinae (Superfamily Strongyloidea) comprises nematodes that are parasitic in the gastrointestinal tracts of macropodid (Family Macropodidae) and vombatid (Family Vombatidae) marsupials. Currently, nine genera and 20 species have been attributed to the subfamily Phascolostrongylinae. Previous studies using sequence data sets for the internal transcribed spacers (ITS) of nuclear ribosomal DNA showed conflicting topologies between the Phascolostrongylinae and related subfamilies. Therefore, the aim of this study was to validate the phylogenetic relationships within the Phascolostrongylinae and its relationship with the families Chabertiidae and Strongylidae using mitochondrial amino acid sequences.

METHODS

The sequences of all 12 mitochondrial protein-coding genes were obtained by next-generation sequencing of individual adult nematodes (n = 8) representing members of the Phascolostrongylinae. These sequences were conceptually translated and the phylogenetic relationships within the Phascolostrongylinae and its relationship with the families Chabertiidae and Strongylidae were inferred from aligned, concatenated amino acid sequence data sets.

RESULTS

Within the Phascolostrongylinae, the wombat-specific genera grouped separately from the genera occurring in macropods. Two of the phascolostrongyline tribes were monophyletic, including Phascolostrongylinea and Hypodontinea, whereas the tribe Macropostrongyloidinea was paraphyletic. The tribe Phascolostrongylinea occurring in wombats was closely related to Oesophagostomum spp., also from the family Chabertiidae, which formed a sister relationship with the Phascolostrongylinae.

CONCLUSION

The current phylogenetic relationship within the subfamily Phascolostrongylinae supports findings from a previous study based on ITS sequence data. This study contributes also to the understanding of the phylogenetic position of the subfamily Phascolostrongylinae within the Chabertiidae. Future studies investigating the relationships between the Phascolostrongylinae and Cloacininae from macropodid marsupials may advance our knowledge of the phylogeny of strongyloid nematodes in marsupials.

摘要

背景

Phascolostrongylinae 亚科（Strongyloidea 超科）包含寄生在袋鼠科（Macropodidae 科）和袋熊科（Vombatidae 科）有袋动物胃肠道中的线虫。目前，该亚科已被归为 9 个属和 20 个种。先前使用核核糖体 DNA 内部转录间隔区（ITS）的序列数据集进行的研究表明，Phascolostrongylinae 亚科与相关亚科之间的拓扑结构存在冲突。因此，本研究旨在使用线粒体氨基酸序列验证 Phascolostrongylinae 内部的系统发育关系及其与 Chabertiidae 和 Strongylidae 科的关系。

方法

通过对代表 Phascolostrongylinae 成员的单个成年线虫（n=8）进行下一代测序，获得了所有 12 个线粒体蛋白编码基因的序列。这些序列被概念性地翻译，并从对齐的、串联的氨基酸序列数据集推断出 Phascolostrongylinae 内部以及与 Chabertiidae 和 Strongylidae 科的关系。

结果

在 Phascolostrongylinae 内部，袋熊特有的属与在有袋动物中出现的属分开。两个 phascolostrongyline 部落是单系的，包括 Phascolostrongylinea 和 Hypodontinea，而 Macropostrongyloidinea 部落是并系的。在袋熊中出现的 Phascolostrongylinea 部落与也来自 Chabertiidae 科的 Oesophagostomum spp. 密切相关，它与 Phascolostrongylinae 形成姐妹关系。

结论

目前的 Phascolostrongylinae 亚科系统发育关系支持先前基于 ITS 序列数据的研究结果。本研究还有助于了解 Phascolostrongylinae 亚科在 Chabertiidae 科中的系统发育位置。未来研究调查来自有袋动物的 Phascolostrongylinae 和 Cloacininae 之间的关系可能会增进我们对有袋动物中 Strongyloid 线虫系统发育的了解。