Jia Wenchao, Xie Danping, Li Fangfang, Wu Xiangzong, Wang Rui, Yang Leifeng, Liu Lijun, Yin Wenhua, Chang Sheng
State Environmental Protection Key Laboratory of Environmental Pollution Health Risk Assessment, South China Institute of Environmental Sciences, Ministry of Ecology and Environment, Guangzhou, 510655, China.
State Environmental Protection Key Laboratory of Environmental Pollution Health Risk Assessment, South China Institute of Environmental Sciences, Ministry of Ecology and Environment, Guangzhou, 510655, China.
Anal Chim Acta. 2021 Oct 23;1183:338976. doi: 10.1016/j.aca.2021.338976. Epub 2021 Aug 21.
Aptamer-functionalized nanoparticles have been widely studied as targeted probes in biomedical applications for targeted therapy and imaging. The rigidity of the nanoparticle could stabilized the spatial structure of the aptamer, ensuring the selectivity and affinity for target recognition in the complex environment. The main aim of this article study was to explore the effect of the spatial structure of aptamer in the interaction between aptamer nanoprobes and receptors. We designed and synthesized aptamer functionalized nanoparticle systems with different derivation lengths, and developed a unique kinetic analysis to quantify affinity interactions. The system used silver decahedral nanoparticles (AgNPs), which was then chemically functionalized with thrombin (or IgE) aptamers of different tail lengths to produced different nanoprobes, and employed thrombin (or IgE) as target on a surface plasmon resonance (SPR) biosensor to evaluate the binding of these nanoprobes. Kinetic analysis of the SPR binding curve was performed to evaluated the affinity between nanoprobes and targets. Under the premise of eliminating multivalent interactions, we found that the distance between aptamer and nanoparticle could affect the affinity between nanoprobe and target. Furthermore, we found that keeping a certain distance between aptamer and nanoparticle could effectively improved the recognition efficiency of the aptamer nanoprobe and target. It shows that the rigidity of nanomaterials could maintain the spatial structure of the aptamer.
适配体功能化纳米颗粒作为靶向探针在生物医学应用中的靶向治疗和成像方面已得到广泛研究。纳米颗粒的刚性可以稳定适配体的空间结构，确保在复杂环境中对靶标识别的选择性和亲和力。本文研究的主要目的是探索适配体空间结构在适配体纳米探针与受体相互作用中的影响。我们设计并合成了具有不同衍生长度的适配体功能化纳米颗粒系统，并开发了一种独特的动力学分析方法来量化亲和相互作用。该系统使用十面体银纳米颗粒（AgNPs），然后用不同尾长的凝血酶（或IgE）适配体对其进行化学功能化，以制备不同的纳米探针，并在表面等离子体共振（SPR）生物传感器上使用凝血酶（或IgE）作为靶标来评估这些纳米探针的结合情况。对SPR结合曲线进行动力学分析以评估纳米探针与靶标之间的亲和力。在消除多价相互作用的前提下，我们发现适配体与纳米颗粒之间的距离会影响纳米探针与靶标之间的亲和力。此外，我们发现保持适配体与纳米颗粒之间的一定距离可以有效提高适配体纳米探针与靶标的识别效率。这表明纳米材料的刚性可以维持适配体的空间结构。
