Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Jiangxi Institute of Gastroenterology and Hepatology, No 17, Yongwai Zheng Street, Nanchang, 330006, Jiangxi, China.
Department of Anorectal Surgery, Chinese People's Liberation Army Joint Security Force Hospital No. 908, No 1028, Jinggangshan Avenue, Nanchang, China.
J Cancer Res Clin Oncol. 2023 Aug;149(10):7895-7903. doi: 10.1007/s00432-023-04646-w. Epub 2023 Feb 21.
Nucleic acid aptamers are a novel molecular recognition tool that is functionally similar to antibodies but superior to antibodies in terms of thermal stability, structural modification, preparation, and cost, and therefore hold great promise for molecular detection. However, due to the limitations of a single aptamer in molecular detection, the multiple aptamer combination for bioanalysis has received much attention. Here, we reviewed the progress of tumor precision detection based on the combination of multiple nucleic acid aptamers and optical methods and discussed its challenges and prospects.
The relevant literature in PubMed was collected and reviewed.
The combination of two or more aptamers with modern nanomaterials and analytical methods allows the fabrication of various detection systems for the simultaneous detection of different structural domains of a substance and/or different substances, including soluble tumor markers, tumor cell surface and intracellular markers, circulating tumor cells, and other tumor-related biomolecules, which has great potential for application in efficient and precise tumor detection.
The combination of multiple nucleic acid aptamers provides a new approach for the precise detection of tumors and will play an important role in precision medicine for tumors.
核酸适体是一种新型的分子识别工具，其功能与抗体类似，但在热稳定性、结构修饰、制备和成本等方面优于抗体，因此在分子检测方面具有很大的应用前景。然而，由于单个适体在分子检测中的局限性，多个适体组合用于生物分析受到了广泛关注。在这里，我们综述了基于多个核酸适体与光学方法相结合的肿瘤精准检测的研究进展，并讨论了其面临的挑战和前景。
检索 PubMed 数据库中相关文献，并进行综述。
将两个或更多的适体与现代纳米材料和分析方法相结合，可以构建各种检测系统，用于同时检测物质的不同结构域和/或不同物质，包括可溶性肿瘤标志物、肿瘤细胞表面和细胞内标志物、循环肿瘤细胞和其他与肿瘤相关的生物分子，在高效、精准的肿瘤检测方面具有很大的应用潜力。
多个核酸适体的组合为肿瘤的精准检测提供了新的途径，将在肿瘤精准医学中发挥重要作用。
