Yunnan University of Traditional Chinese Medicine, Kunming, 650500, China.
Yunnan Institute of Tobacco Quality Inspection and Supervision, Kunming, 650106, China.
J Ethnopharmacol. 2022 Jan 30;283:114484. doi: 10.1016/j.jep.2021.114484. Epub 2021 Oct 7.
The diaphragma juglandis (DJ) comes from the wooden septum in the core of Juglans regia L, also known as the walnut septum. In Iranian traditional medicine, walnut distraction wood was widely used in the treatment of diabetes. However, there is a lack of research data on the mechanism of DJ against diabetes.
To explore the protective effect of diaphragma juglandis extract (DJE) on type 2 diabetic rats and the hypoglycemic mechanism of DJE.
Supplemented DJE and fed a high-fat diet for five weeks, and then injected low-dose STZ, successfully induced type 2 diabetic rats. Collected rat serum, liver, pancreas and feces to determine the biochemical parameters of serum and liver, analyze the pathological damages of pancreas and liver, and measure the changes of gut microbes in feces.
DJE could inhibit the metabolic abnormalities of T2DM by improving insulin resistance, abnormal lipid metabolism, liver damage, oxidative stress, and reducing inflammation. DJE significantly held fasting blood glucose, glycosylated serum protein, serum low density lipoprotein, high density lipoprotein, oral glucose tolerance test, nitric oxide, superoxide dismutase and catalase, serum and liver triglycerides, total cholesterol, aspartate aminotransferase, alanine aminotransferase, malondialdehyde, lipopolysaccharide, fasting insulin and tumor necrosis factor-α and prevented the pathological damage of pancreas and liver. The 16SrRNA gene sequencing results showed that DJE intercepted the disorders of the fecal gut microbes, mainly including Lactobacillaceae, Rikenella, Pygmaiobacter, Oscillospiraceae and Klebsiella. Spearman correlation analysis showed that the changes of gut microbes were closely relative with biochemical parameters.
DJE might prevent type 2 diabetes and its complications and hold up the disorders of gut microbes.
胡桃隔来源于胡桃科胡桃属植物胡桃 Juglans regia L 的木质隔膜，又名隔木。在伊朗传统医学中，分心木被广泛用于治疗糖尿病。然而，关于胡桃隔防治糖尿病的作用机制，目前缺乏研究数据。
探讨胡桃隔提取物（DJE）对 2 型糖尿病大鼠的保护作用及其降血糖机制。
采用高脂饲料喂养并补充 DJE 五周，然后注射小剂量链脲佐菌素（STZ），成功诱导 2 型糖尿病大鼠。收集大鼠血清、肝脏、胰腺和粪便，测定血清和肝脏生化参数，分析胰腺和肝脏的病理损伤，并检测粪便中肠道微生物的变化。
DJE 通过改善胰岛素抵抗、异常脂质代谢、肝损伤、氧化应激和减轻炎症，抑制 T2DM 的代谢异常。DJE 能显著降低空腹血糖、糖化血清蛋白、血清低密度脂蛋白、高密度脂蛋白、口服葡萄糖耐量试验、一氧化氮、超氧化物歧化酶和过氧化氢酶、血清和肝脏甘油三酯、总胆固醇、天门冬氨酸氨基转移酶、丙氨酸氨基转移酶、丙二醛、脂多糖、空腹胰岛素和肿瘤坏死因子-α，防止胰腺和肝脏的病理损伤。16SrRNA 基因测序结果表明，DJE 阻断了粪便肠道微生物的紊乱，主要包括乳杆菌科、Rikenella、Pygmaiobacter、Oscillospiraceae 和克雷伯氏菌。Spearman 相关分析表明，肠道微生物的变化与生化参数密切相关。
DJE 可能预防 2 型糖尿病及其并发症，并纠正肠道微生物紊乱。
