Department of Biological Sciences, Faculty of Natural Sciences.
African Centre of Excellence for Genomics of Infectious Diseases (ACEGID), Redeemer's University, Ede, Osun State, Nigeria.
AIDS. 2022 Jan 1;36(1):137-146. doi: 10.1097/QAD.0000000000003098.
This study was designed to provide information on the genetic diversity of HIV-1 and drug resistance mutations in Nigeria, as there is limited understanding of variants circulating in the country.
We used an advanced next-generation sequencing platform, Primer ID, to: investigate the presence of high and low abundance drug resistance mutations; characterize preexisting Integrase Strand Transfer Inhibitor (INSTI) mutations in antiretroviral therapy (ART)-experienced but dolutegravir-naive individuals; detect recent HIV-1 infections and characterize subtype diversity from a cohort of people with HIV-1 (PWH).
HIV-1 subtype analysis revealed the predominance of CRF02_AG and subtype G in our study population. At detection sensitivity of 30% abundance, drug resistance mutations (DRMs) were identified in 3% of samples. At a sensitivity level of 10%, DRMs were identified in 27.3% of samples. We did not detect any major INSTI mutation associated with dolutegravir-resistance. Only one recent infection was detected in our study population.
Our study suggests that dolutegravir-containing antiretroviral regimens will be effective in Nigeria. Our study also further emphasizes the high genetic diversity of HIV-1 in Nigeria and that CRF02_AG and subtype G are the dominant circulating forms of HIV-1 in Nigeria. These two circulating forms of the virus are largely driving the epidemic in the country.
本研究旨在提供尼日利亚 HIV-1 遗传多样性和耐药突变的信息，因为人们对该国流行的变异体了解有限。
我们使用先进的下一代测序平台 Primer ID：调查高和低丰度耐药突变的存在；描述抗逆转录病毒治疗 (ART) 经验丰富但多拉韦林初治个体中预先存在的整合酶链转移抑制剂 (INSTI) 突变；从一组 HIV-1 患者 (PWH) 中检测近期 HIV-1 感染并描述亚型多样性。
HIV-1 亚型分析显示，我们的研究人群中以 CRF02_AG 和亚型 G 为主。在 30%丰度的检测灵敏度下，3%的样本中发现耐药突变 (DRMs)。在灵敏度水平为 10%时，27.3%的样本中发现了 DRMs。我们没有发现任何与多替拉韦耐药相关的主要 INSTI 突变。我们的研究人群中仅检测到一例近期感染。
我们的研究表明，含多替拉韦的抗逆转录病毒方案将在尼日利亚有效。我们的研究还进一步强调了尼日利亚 HIV-1 的遗传多样性很高，CRF02_AG 和亚型 G 是尼日利亚流行的 HIV-1 主要循环形式。这两种循环形式的病毒在很大程度上推动了该国的疫情。
