Department of Medical Aesthetics, Linyi Central Hospital, Linyi, 276400, Shandong, China.
Department of Burn Plastic Surgery, Binzhou Medical University Hospital, No. 661 Huanghe 2nd Road, Binzhou, 256603, Shandong, China.
Arch Dermatol Res. 2022 Oct;314(8):759-766. doi: 10.1007/s00403-021-02285-0. Epub 2021 Oct 10.
Melanoma is one of the most common malignant tumors that originate from nerve sheath melanocytes and are produced in the skin and other organs. This paper mainly studied the role of miR-582 in melanoma. The expression of miR-582 in melanoma cell lines and tissues was evaluated by real-time quantitative PCR. Kaplan-Meier curve and Cox proportional hazards model analysis were used to investigate the prognostic value of miR-582 in melanoma. Cell proliferation, invasion, and migration were analyzed using CCK-8 and transwell assays. The results showed that miR-582 was significantly decreased in melanoma cells and tissues. Downregulation of miR-582 was associated with ulceration, lymph node metastasis, TNM stage, and poor overall survival. The functional results showed that low expression of miR-582 can promote cell proliferation, migration, and invasion. FOXC1 was a direct target of miR-582. Overall, the expression of miR-582 is downregulated in melanoma tissues and cell lines. Low expression of miR-582 is associated with prognosis and progression of melanoma by targeting FOXC1. miR-582 may be a prognostic biomarker and a new therapeutic strategy for melanoma.
黑色素瘤是最常见的恶性肿瘤之一，起源于神经鞘黑色素细胞，产生于皮肤和其他器官。本文主要研究了 miR-582 在黑色素瘤中的作用。通过实时定量 PCR 评估了 miR-582 在黑色素瘤细胞系和组织中的表达。采用 Kaplan-Meier 曲线和 Cox 比例风险模型分析 miR-582 在黑色素瘤中的预后价值。通过 CCK-8 和 Transwell 检测分析细胞增殖、侵袭和迁移。结果表明，miR-582 在黑色素瘤细胞和组织中显著降低。miR-582 的下调与溃疡、淋巴结转移、TNM 分期和总体生存不良有关。功能结果表明，miR-582 低表达可促进细胞增殖、迁移和侵袭。FOXC1 是 miR-582 的直接靶标。总的来说，miR-582 在黑色素瘤组织和细胞系中的表达下调。miR-582 的低表达通过靶向 FOXC1 与黑色素瘤的预后和进展相关。miR-582 可能是黑色素瘤的预后生物标志物和新的治疗策略。
