Wang Lujun, Pumera Martin
Future Energy and Innovation Laboratory, Central European Institute of Technology, Brno University of Technology (CEITEC-BUT), 61200, Brno, Czech Republic.
School of Pharmacy, Southwest Medical University, Luzhou, Sichuan, 646000, People's Republic of China.
Mikrochim Acta. 2021 Oct 10;188(11):374. doi: 10.1007/s00604-021-05006-6.
Three-dimensional (3D) printing has showed great potential for the construction of electrochemical sensor devices. However, reported 3D-printed biosensors are usually constructed by physical adsorption and needed immobilizing reagents on the surface of functional materials. To construct the 3D-printed biosensors, the simple modification of the 3D-printed device by non-expert is mandatory to take advantage of the remote, distributed 3D printing manufacturing. Here, a 3D-printed electrode was prepared by fused deposition modeling (FDM) 3D printing technique and activated by chemical and electrochemical methods. A glucose oxidase-based 3D-printed nanocarbon electrode was prepared by covalent linkage method to an enzyme on the surface of the 3D-printed electrode to enable biosensing. X-ray photoelectron spectroscopy and scanning electron microscopy were used to characterize the glucose oxidase-based biosensor. Direct electrochemistry glucose oxidase-based biosensor with higher stability was then chosen to detect the two biomarkers, hydrogen peroxide and glucose by chronoamperometry. The prepared glucose oxidase-based biosensor was further used for the detection of glucose in samples of apple cider. The covalently linked glucose oxidase 3D-printed nanocarbon electrode as a biosensor showed excellent stability. This work can open new doors for the covalent modification of 3D-printed electrodes in other electrochemistry fields such as biosensors, energy, and biocatalysis.
三维（3D）打印在电化学传感器装置的构建方面已显示出巨大潜力。然而，已报道的3D打印生物传感器通常通过物理吸附构建，且需要在功能材料表面固定试剂。为了构建3D打印生物传感器，非专业人员对3D打印设备进行简单改性对于利用远程分布式3D打印制造至关重要。在此，通过熔融沉积建模（FDM）3D打印技术制备了3D打印电极，并通过化学和电化学方法进行活化。通过共价连接法将基于葡萄糖氧化酶的3D打印纳米碳电极制备在3D打印电极表面的酶上，以实现生物传感。采用X射线光电子能谱和扫描电子显微镜对基于葡萄糖氧化酶的生物传感器进行表征。然后选择稳定性更高的基于直接电化学葡萄糖氧化酶的生物传感器，通过计时电流法检测两种生物标志物过氧化氢和葡萄糖。所制备的基于葡萄糖氧化酶的生物传感器进一步用于检测苹果酒样品中的葡萄糖。作为生物传感器的共价连接葡萄糖氧化酶3D打印纳米碳电极显示出优异的稳定性。这项工作可为生物传感器、能源和生物催化等其他电化学领域的3D打印电极的共价修饰打开新的大门。
