Institute of Organic Chemistry and Biochemistry, The Czech Academy of Sciences, Prague, Czechia.
Adv Exp Med Biol. 2021;1336:87-104. doi: 10.1007/978-3-030-77252-9_5.
Peptides play a crucial role in many vitally important functions of living organisms. The goal of peptidomics is the identification of the "peptidome," the whole peptide content of a cell, organ, tissue, body fluid, or organism. In peptidomic or proteomic studies, capillary electrophoresis (CE) is an alternative technique for liquid chromatography. It is a highly efficient and fast separation method requiring extremely low amounts of sample. In peptidomic approaches, CE is commonly combined with mass spectrometric (MS) detection. Most often, CE is coupled with electrospray ionization MS and less frequently with matrix-assisted laser desorption/ionization MS. CE-MS has been employed in numerous studies dealing with determination of peptide biomarkers in different body fluids for various diseases, or in food peptidomic research for the analysis and identification of peptides with special biological activities. In addition to the above topics, sample preparation techniques commonly applied in peptidomics before CE separation and possibilities for peptide identification and quantification by CE-MS or CE-MS/MS methods are discussed in this chapter.
肽在许多生命活动中起着至关重要的作用。肽组学的目标是鉴定“肽组”，即细胞、器官、组织、体液或生物体中的全部肽类物质。在肽组学或蛋白质组学研究中，毛细管电泳（CE）是液相色谱的替代技术。它是一种高效快速的分离方法，仅需极少量的样品。在肽组学方法中，CE 通常与质谱（MS）检测相结合。最常见的是 CE 与电喷雾电离 MS 联用，较少与基质辅助激光解吸/电离 MS 联用。CE-MS 已被广泛应用于研究不同体液中各种疾病的肽类生物标志物，或用于食品肽组学中具有特殊生物活性的肽类的分析和鉴定。除了上述主题外，本章还讨论了 CE 分离前在肽组学中常用的样品制备技术，以及通过 CE-MS 或 CE-MS/MS 方法进行肽鉴定和定量的可能性。
