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近年来在提高毛细管和微芯片电泳和电色谱灵敏度方面的进展(2016-2018 年)。

Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2016-2018).

机构信息

Australian Centre for Research on Separation Science, Chemistry, School of Natural Science, University of Tasmania, Hobart, Tasmania, Australia.

Department of Biopharmaceutics and Pharmacodynamics, Medical University of Gdansk, Gdansk, Poland.

出版信息

Electrophoresis. 2019 Jan;40(1):17-39. doi: 10.1002/elps.201800384. Epub 2018 Nov 6.

Abstract

One of the most cited limitations of capillary and microchip electrophoresis is the poor sensitivity. This review continues to update this series of biannual reviews, first published in Electrophoresis in 2007, on developments in the field of online/in-line concentration methods in capillaries and microchips, covering the period July 2016-June 2018. It includes developments in the field of stacking, covering all methods from field-amplified sample stacking and large-volume sample stacking, through to isotachophoresis, dynamic pH junction, and sweeping. Attention is also given to online or in-line extraction methods that have been used for electrophoresis.

摘要

毛细管电泳和微芯片电泳最常被引用的局限性之一是灵敏度差。本综述继续更新这一系列每两年一次的综述,该综述最初于 2007 年在电泳杂志上发表,内容涉及 2016 年 7 月至 2018 年 6 月毛细管和微芯片中在线/在线浓缩方法领域的发展。它包括堆积领域的发展,涵盖了从场放大样品堆积和大体积样品堆积到等速电泳、动态 pH 结和扫集的所有方法。同时还关注了用于电泳的在线或在线提取方法。

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